摘要
目的研究栀子苷的最佳超声提取条件。方法采用响应面分析法优化栀子苷的超声提取条件,在单因素实验基础上利用BBD组合设计Box-Behnken中心组合设计拟合试验条件。结果以栀子苷提取率为响应值进行多元二次响应回归分析,可得超声提取栀子苷的最佳工艺条件为:超声功率750.87 W、提取时间46.57 min、溶剂浓度52.06%、溶剂体积(料液比)1:64.09 g·mL-1。此最优条件下栀子苷提取率预测为36.16 mg·g-1,验证值为35.49 mg·g-1,与预测值的相对误差为1.85%。结论此最优工艺条件具有一定的实际应用价值,对工业化生产有一定的指导意义。
Objective To study the technology of extracting geniposide from Gardenia jasminoides Ellis by ultrasound extraction. Methods Based on the single-factor experirnents, a four-factor-three-level experiment design was developed by Box-Behnken central composite design method. Multi-quadratic equation was established with the yield of geniposide as response values. Results The optimum extraction condition was obtained as follows： ultrasound power 750.87 W, extraction time 46.57 min, ethanol concentration 52.06 % and ethanol volume（ratio of solvent to solid） 1：64.09 g ·mL^-1 The predictive maximum extraction yield was 36.16 mg ·mL^-1 while the average experimental value was 35.49 mg·mL^-1, with the relative error of 1.85%. Conclusion The method is reliable, and can be used for industrial production.
出处
《中国药物警戒》
2011年第3期154-157,共4页
Chinese Journal of Pharmacovigilance
关键词
栀子苷
超声提取
响应曲面分析法
geniposide
ultrasound extraction
response surface methodology
