摘要
采用高效液相色谱法对槟榔中的多酚类物质进行分析。用甲醇提取槟榔中的多酚类物质,并依次用石油醚、乙酸乙酯、正丁醇萃取,萃取物经抽真空浓缩,流动相定容,高效液相色谱法测定。分析结果表明:槟榔幼果较槟榔成熟果中所含的多酚种类和数量少,槟榔成熟果中果仁的多酚种类和数量都远较皮中多;槟榔的甲醇提取物乙酸乙酯萃取部分中所含的多酚种类和数量相比石油醚和正丁醇萃取部分都是最多的;槟榔仁的多酚中含量最多的是儿茶素,含量为1610 mg/kg,其次是单宁酸622 mg/kg,表没食子儿茶素228 mg/kg,表儿茶素164 mg/kg,表没食子儿茶素没食子酸酯72 mg/kg,没食子酸13.6 mg/kg。而没食子儿茶素、绿原酸、和没食子儿茶素没食子酸酯没有检测到。
HPLC was used to determine polyphenols in Areca catechu. Polyphenols were extracted from betel nut with methanol, which were extracted with petroleum ether, ethyl acetate, and n-butanol, successively. Then, the extracts were concentrated and determined by HPLC after making constant volume using the mobile phase. The results showed that the kinds and quantities of polyphenols in mature young betel nut were fewer than in mature fruit, while those in nuts of ma2 ture fruit were more than in the skin. The kinds and quantities of polyphenols in the methanol extract were the most. The content of catechin was the highest reaching 1610 mg/kg in the polyphenols, tannic acid （622 mg./kg）, epigallocatechin （228 mg/kg） ,epicatechin （164 mg/kg） ,epigallocatechin gallate （72 mg/kg） ,and gallic acid （13.6 mg/kg） were followed. However, catechin gallate, chloregenic acid, and gallocatechin gallate were not detected.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第1期101-104,共4页
Natural Product Research and Development
基金
2008年中央级公益性科研院所基本科研业务费(ITB-BKF083)
