摘要
为了掌握朱鹮禽流感病毒流行情况,利用鸡胚分离方法从病死朱鹮脑组织标本中分离出禽流感病毒毒株,并将所分离的病毒进行血凝试验、血凝抑制试验、鸡胚最小致死量平均死亡时间(MDT)、半数致死量(LD50)及1日龄鸡脑内接种致病指数(ICPI)试验。结果表明,所分离的病毒均能够凝集鸡、肉鸭、绵羊、山羊、猪、人、兔等的红细胞,且这种血凝性可被H9型标准抗体血清所抑制,所分离毒株的MDT为48 h,LD50指数为50%,ICPI为1.544。以上结果表明所分离的病毒为低致病力禽流感毒株。
To isolate and identify avian influenza virus in crested ibis（Nipponian nippon）,specimens were collected from dead crested ibis.The suspension of brain tissue of crested ibis was used to inoculate SPF chicken embryo,then the AIV was isolated.Hemagglutination test（HA）,hemagglutination inhibition（HI）,the death time of chicken embryos（MDT）,median lethal dosage（LD50） and the intracerebral pathogenicity index（ICPI） tests were carried out respectively to identify the serotype of AIV.The strain could agglutinate the erythrocytes of chicken,duck,sheep,goat,swine,human and rabbit.The hemagglutination of viruses could be inhibited by H9N2 antiserum.The MDT of isolated strain was 48 h,index of LD50 was 50%,and ICPI was 1.544.The results showed that the serotype of this strain was identified as H9,which belonged to LPAIV.This strain of AIV derived from crested ibis was LPAIV,and its virulence was very weak.
出处
《动物医学进展》
CSCD
北大核心
2011年第2期42-44,共3页
Progress In Veterinary Medicine
基金
陕西省自然科学研究计划项目(2004C1-25)
关键词
朱鹮
禽流感病毒
分离鉴定
crested ibis
Avian influenza virus
isolation and identification
