摘要
目的测定不同产地黄连药材加工品中盐酸药根碱、盐酸非洲防己碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀、盐酸小檗碱的含量,为黄连药材的真伪鉴别提供依据。方法色谱条件为Phenomenex Gemini-NX C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-30 mmol.L-1碳酸氢铵溶液(每1 000 mL碳酸氢铵溶液含7 mL氨水,1 mL三乙胺)梯度洗脱;流速1.0 mL.min-1;检测波长270 nm;柱温30℃。结果黄连药材中6个生物碱含量均较高,且其中盐酸药根碱、盐酸防己碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀和盐酸小檗碱的含量分别不低于0.30%,0.40%,1.10%,2.02%,1.45%,6.58%。结论方法准确、可行,可作为黄连药材质量的定量依据。
OBJECTIVE To determine the contents of jateorrhizine hydrochloride,columbamine hydrochloride,epiberberine hydrochloride,coptisine hydrochloride,palmatine hydrochloride,and berberine hydrochloride in crude Coptidis Rhizoma and explore the quality relationship of Coptidis Rhizoma,which providing a basis for distinguishing.METHODS The samples were separated by a Phenomenex Gemini-NX C18 column（250 mm×4.6 mm,5 μm） using acetonitrile-30 mmol·L.1 ammonium bicarbonate（each 1 000 mL ammonium bicarbonate contained 7 mL ammonia water and 1 mL triethylamine） gradient system as mobile phase at the flow rate of 1.0 mL·min.1,the detective wavelength was 270 nm and the column temperature was 30 ℃.RESULTS The quality of Coptidis Rhizoma from different areas is high,the lowest average contents of jateorrhizine hydrochloride,columbamine hydrochloride,epiberberine hydrochloride,coptisine hydrochloride,palmatine hydrochloride,and berberine hydrochloride in Coptidis Rhizoma are 0.30%,0.40%,1.10%,2.02%,1.45%,6.58%.CONCLUSION This method is rapid,accurate,sensitive,reproducible.It is proved to be suitable for the quality control of Coptidis Rhizoma.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第2期128-133,共6页
Chinese Journal of Modern Applied Pharmacy
基金
中医药行业科研专项(200707014)
