摘要
目的:探讨DNA甲基转移酶1(DNMT1)基因在喉鳞状细胞癌中的表达及其与临床病理因素的关系。方法:采用免疫组织化学方法检测96例喉鳞状细胞癌中DNMT1基因蛋白的表达,其中66例喉癌组织同时进行实时定量PCR,并分析其DNMT1mRNA的表达与临床病理因素的关系。结果:DNMT1mRNA在喉鳞状细胞癌组织中表达上调(P<0.05)。96例喉癌鳞状上皮细胞中DNMT1蛋白表达全部为阳性(96/96),36%(8/22)癌旁组织的鳞状上皮细胞中DNMT1蛋白表达为阳性,二者差异有统计学意义(P<0.05)。DNMT1mRNA在喉癌组织中表达上调,和患者年龄、性别、临床分期、淋巴结转移情况无关,但与患者吸烟史有显著相关性(P<0.05)。结论:DNMT1可能在喉癌中表达增高从而引起肿瘤的发生,吸烟可能诱导DNMT1的表达增高。
Objective：To investigate the expression of DNMT1 in laryngeal squamous cell carcinoma （LSCC）and its relationship with clinicopathological factors.Method：Ninety-six cases of LSCC samples were detected by immunohistochemical staining to analyze the expression of DNMT1 protein,while 66 cases of them were detected by real-time PCR.And the relationship between the expression of DNMT1 mRNA and clinicopathologic factors was then analyzed.Result：The expression of DNMT1 mRNA in LSCC were up-regulated（P0.05）.The positive rate of DNMT1 protein expression in LSCC and in peri-cancer tissue were 100%（96/96）and 36%（8/22） respectively,which showed a significant difference（P0.05）.DNMT1 mRNA expression wasn＇t correlated with patients＇ age,gender,T stage and lympha node metastasis,but was associated with smoking habit（P0.05,respectively）.Conclusion：DNMT1 may initiate the oncogenesis of LSCC by increasing expression,and smoking habit may induce the expression of DNMT1 gene.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第24期1105-1107,1111,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
