摘要
目的研究生长因子抑制剂苏拉明对外伤性增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)玻璃体中生长因子的影响及防治作用。方法将40只健康新西兰大白兔随机平均分为5组,除空白组外,其余均制备外伤性PVR模型。然后分别将生理盐水(0.1mL)和浓度分别为40g.L-1、60g.L-1和80g.L-1苏拉明(0.1mL)注入模型组和实验1、2、3组玻璃体腔内,空白组不进行注射。对眼底连续观察28d并对PVR进行分级,采用双抗夹心ELISA法和放射免疫方法分别检测玻璃体液中肿瘤坏死因子-α(tumor necrosis factorα,TNF-α)和表皮生长因子(epidermal growth factor,EGF)的含量。结果造模后第28天,空白组、模型组及实验1、2、3组玻璃体腔中TNF-α含量分别为(0.245±0.346)μg.L-1、(3.984±0.302)μg.L-1、(3.024±0.163)μg.L-1、(2.038±0.239)μg.L-1、(1.285±0.151)μg.L-1;EGF含量分别为(0.180±0.385)μg.L-1、(1.025±0.092)μg.L-1、(0.540±0.073)μg.L-1、(0.274±0.069)μg.L-1、(0.242±0.322)μg.L-1。实验1、2、3组和空白组玻璃体腔中TNF-α和EGF含量均低于模型组,差异均有显著统计学意义(均为P<0.01)。实验1、2、3组玻璃体腔中TNF-α的含量均高于空白组,差异均有统计学意义(P<0.01或P<0.05)。实验1组玻璃体腔中EGF的含量高于空白组,差异有显著统计学意义(P<0.01)。实验2、3组玻璃体腔中EGF的含量与空白组比较,差异无统计学意义(P>0.05)。实验2、3组玻璃体腔中TNF-α和EGF含量均低于实验1组,差异均有统计学意义(均为P<0.05)。形态学上未发现明显视网膜毒副作用。结论苏拉明玻璃体腔注射安全有效,通过降低玻璃体中细胞因子TNF-α和EGF的含量,有效抑制了兔外伤性PVR的发展,为以后临床应用提供可靠的理论依据。
Objective To investigate the experimental therapeutic effects of suramin on growth factors in traumatic proliferative vitreoretinopathy vitreous body.Methods Forty New zealand white rabbits were selected and randomly divided into 5 groups,and they were induced to traumatic PVR models except blank group.The eyes of model group and experiment group 1,group 2,group 3 respectively received an intravitreal injection of saline（0.1 mL）and suramin（0.1 mL）in different concentrations of 40 g·L-1,60 g·L-1 and 80 g·L-1,the blank group received nothing.The ocular fundus were continuously observed for 28 days and divided in different stages.The contents of epidermal growth factor（EGF）and tumor necrosis factor α（TNF-α）were measured by Double-Antibody Sandwich ELISA and radio-immunity assay.Results The contents of TNF-α in blank group,model group and experiment group 1,group 2 and group 3 were（0.245±0.346）μg·L-1,（3.984±0.302）μg·L-1,（3.024±0.163）μg·L-1,（2.038±0.239）μg·L-1,（1.285±0.151）μg·L-1 and the contents of EGF were（0.180±0.385）μg·L-1,（1.025±0.092）μg·L-1,（0.540±0.073）μg·L-1,（0.274±0.069）μg·L-1,（0.242±0.322）μg·L-1 on the 28th day after modeling.The contents of TNF-α and EGF in experiment group 1,group 2 and group 3 were lower than that in model group,there were significant differences（all P0.01）.The contents of TNF-α in experiment group 1,group 2 and group 3 were higher than that in blank group,the differences were statistically significant（P0.01 or P0.05）.The contents of EGF in experiment group 1 were higher than that in blank group,there was significant difference（P0.01）.The contents of EGF in experiment group 2 and group 3 were not significant compared with blank group（P0.05）.The contents of TNF-α and EGF in experiment 2 and group 3 were lower than that in experiment group 1,the differences were statistically significant（all P0.05）.There was no obvious morphological retinal toxicity.Conclusions Intravitreal inj
出处
《眼科新进展》
CAS
北大核心
2011年第2期119-122,126,共5页
Recent Advances in Ophthalmology
