摘要
目的:探讨上皮-间质转化(EMT)及其转录调控因子Twist1在人胚胎前列腺发育过程中的作用及意义。方法:应用免疫组化方法研究EMT的标志性蛋白E-Cadherin、N-Cadherin、Vimentin及转录调控因子Twist1在不同胎龄(25例,16～39周)人前列腺组织中的表达变化情况,分析其与胚胎前列腺发育的关系。结果:E-Cadherin仅表达于前列腺腺体,Vimentin、N-Cadherin和Twist1在腺体和腺体周围间质细胞中均有表达。随胎龄增大,腺体E-Cadherin的表达水平逐渐增强,而Vimentin、N-Cadherin和Twist1的表达水平则逐渐减弱。间质表达的Vimentin、N-Cadherin和Twist1在整个过程中无明显变化。结论:人胚胎前列腺发育过程中存在EMT现象,在早期可能有助于前列腺上皮细胞的游走迁移,以形成腺管;后期则可能对上皮细胞的分化起重要调控作用。
Objective：To investigate the role and significance of epithelial-mesenchymal transition （EMT） and its transcriptional regulator Twist1 in the development of the human fetal prostate.Methods： Twenty-five human fetal prostate specimens at various developmental stages （16-39 weeks） were included in this study.EMT markers,such as E-Cadherin,N-Cadherin and Vimentin,and EMT transcriptional regulator Twist1 were determined by immunohistochemistry,and their relationship with the development of the human fetal prostate was analyzed.Results： E-Cadherin was expressed in the fetal prostate epithelium only,while Vimentin,N-Cadherin and Twist1 in both the epithelium and the stroma.The expression of E-Cadherin gradually increased,but those of Vimentin,N-Cadherin and Twist1 gradually decreased with the gestation stages.No significant changes were observed in the staining patterns of Vimentin,N-Cadherin and Twist1 in the stroma during the whole developmental process.Conclusion： EMT is involved in the development of the human fetal prostate,which may promote epithelial cell motility to form prostatic bud tubules in early gestation stages and boost the differentiation of prostate epithelia in later stages.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2011年第2期146-150,共5页
National Journal of Andrology
