摘要
在深圳大鹏澳海水鱼类网箱养殖区取得表层沉积物,通过2个月的间歇曝气选择性富集,分离出6株细菌,通过测定其对野生杂鱼鱼糜饵料有机物的降解能力,进一一步筛选出4株对鱼糜饵料有机物有快速、高效降解能力的细菌。7 d培养生化需氧量(BOD_7)范围在1 040～1 140 mg/L,5 d培养化学需氧量去除率(COD_(Mn))范围在13.58%～46.9%,有机物的可生化降解性(BOD_5/COD_0)在81.56%～89.43%。4株细菌两两组合的5 d培养CODM_(Mn)平均去除率为64.91%±6.51%,是单株菌平均去除率(30.60%±13.63%)的2倍多;而BOD_5/COD_0范围在86.10%～89.13%,与单株菌间没有明显差别。通过部分长度16S rDNA序列分析,并与GenBank和EMBL数据进行同源性检索,结果表明,有2株细菌分别隶属于表皮葡萄球菌属(Staphylo-coccus sp.)和盐单胞菌属(Halomonas sp.),另2株分别与盐单胞菌属(Halomonas sp.)和假单胞菌属(Pseudomonas sp.)相近。
This paper is aimed to present our study on the screening and identification of effective organic pollutant degrading bacteria in the sediment of a heavily organic polluted marine cage fish farm. As a matter of fact, the marine cage fish farming first began in Guangdong coastal waters of China at the end of 1970s, and it has grown dramatically during the last three decades and become one of the important marine aquaculture industries in China. However, marine cage fish farming generates high pollution loadings especially in the sea bottom, where the high sediment oxygen demand, anoxic sediments, production of toxic gases and decrease in benthic diversity may result. For bioremediation of polluted sediment environment in marine cage farm, six bacteria strains were isolated after 2 months of selective enrichment incubation under intermittent aeration condition from the sediment in the marine cage fish farm in Dapeng Ao Cove, east coast of Shenzhen City. Through the ability test for degradation to the liquid wild trash fish culture medium, four bacteria strains which were capable of degrading the organic trash fish pollutant rapidly and efficiently, were obtained. The 7 d incubation of biochemical oxygen demand （BOD7） were between 1 040 - 1 140 mg/L, the 5 d incubation for the average CODM, removing rate （ 1 - COD5/COD0） were between 13.58% - 46.9% and the biochemical degradation rate （BOD5/COD0） were between 81.56% - 89.43% . The 5 d incubation for average CODM, degradation rate of pair-strain mixed bacteria was64.91% （6.51%, which was 2 times higher than that 30.60 （13.63% of single strain; and BODs/COD0 were between 86.10% -89.13%. There were no obvious differences if compared with those of single strain. Sequence analysis based on partial 16S rDNA and performed by BLASTN and FASTA showed that 2 strains belonged to genus of Staphylococcus sp. and Halomonas sp. The other 2 strains maybe belonged to genus of Halomonas sp. and Pseudomonas sp., respectively.
出处
《安全与环境学报》
CAS
CSCD
北大核心
2011年第1期55-59,共5页
Journal of Safety and Environment
基金
中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)项目(2007ZD07)
广东省科技计划项目(2006A36502003
2006B60202026
2007A032600004)
广东省海洋渔业科技推广专项项目(A200899J01)
十一五国家科技支撑计划项目(2006BAD09A14)
关键词
水产养殖学
海水网箱养殖
有机污染物降解菌
筛选
细菌鉴定
marine cage fish farming
organic pollutant degradingbacteria
screening
bacterial identification
