摘要
二倍体刺槐(diploid Robinia pseudoacacia)是我国水土保持林的先锋树种,具有较强的适应性和抗逆性,对改善生态环境、防治水土流失、调节水文状况有重要作用。四倍体刺槐(tetraploid Robinia pseudoacacia)是二倍体刺槐的加倍品种,也称多倍体刺槐,由韩国引进,具有速生、耐盐碱、耐干旱和耐烟尘等特点。目前,关于四倍体刺槐的研究,主要集中于栽培技术和繁殖技术方面,而关于四倍体刺槐叶片超微结构与其耐盐性的关系尚缺乏报道。比较了二倍体刺槐和四倍体刺槐在NaCl和Na2SO4胁迫下,叶片叶绿体超微结构的变化特点,一方面可以对二者的耐盐性进行鉴定,同时也可以探讨不同盐分胁迫条件下的作用机制。利用NaCl和Na2SO4进行20d的盐胁迫处理,观察叶绿体超微结构的变化特点,发现:NaCl处理前,二者叶肉细胞叶绿体为梭形、形态饱满、结构完整,NaCl处理后10d时,二倍体刺槐的叶绿体出现变形、膜模糊、基粒片层松散、类囊体解体、脂质球增多等现象,NaCl处理后20d时,叶绿体肿胀、变形,基粒片层断裂,膜系统解体。Na2SO4处理后10d时,二倍体刺槐的叶绿体肿胀,膜模糊,基粒片层松散、类囊体解体,Na2SO4盐胁迫处理后20d时,膜系统全部解体,结构破坏更为严重。总体来说,四倍体刺槐在盐胁迫后叶绿体结构变化不明显,只是在Na2SO4处理20d时,四倍体刺槐的叶绿体出现中空、基粒片层松散、膜边缘模糊现象。在处理前,两种刺槐的叶绿体均紧贴细胞壁,分布于细胞壁边缘。在NaCl处理后10d时,二倍体刺槐的叶绿体仍呈有序排列,紧贴细胞壁,但在处理后20d时,大部分叶绿体脱离细胞壁,呈随机分布。在Na2SO4处理后10d时,二倍体刺槐部分叶绿体脱离细胞壁,位于细胞中央。在Na2SO4处理后20d时,二倍体刺槐叶绿体大部分与细胞壁脱离。四倍体刺槐在两种盐胁迫处理前后叶绿体的排列变化不明显,均分
The diploid black locust（diploid Robinia pseudoacacia） is a pioneer species of China′s soil and water conservation forest,has strong adaptability and resistance and plays an important role in improving the ecological environment,preventing soil erosion and regulating hydrological conditions.The tetraploid black locust,also known as the polyploid black locust,is a double species of the diploid black locust.It was introduced from South Korea,with fast-growing,saline-alkali tolerant,drought-tolerant,dust-resistant and other characteristics.At present,studies on the tetraploid black locust focus mainly on cultivation and breeding techniques,however,the relationship between the ultrastructure of its leaves and its salt tolerance is still a lack of coverage.This study compared the changes in the leaf chloroplast ultrastructures of the diploid and tetraploid black locust under NaCl and Na2SO4 stress,which can not only identify their salt tolerance but also explore the function mechnism of salt stress to different plants. The ultramicrostructure of the chloroplast in the tetraploid diploid black locust was observed under NaCl and Na2SO4 stress during 20 d.Before NaCl treatment,its chloroplast appeared spindle in shape,full in form and complete in structure.After NaCl treatment for 10 d,the chloroplast of the diploid black locust showed distortion,ambiguity in membrane,loose in grana lamella,disintegration in thylakoid,increases in lipid ball and so on.After NaCl stress for 20 d,the chloroplast of the diploid black locust displayed swelling and distortion,fracture in grana lamella and disintegration in membrane system.After Na2SO4 stress for 10 d,the chloroplast of the diploid black locust exhibited swelling,ambiguity in membrane,loose in grana lamella,disintegration in thylakoid.After Na2SO4 stress for 20 d,all membrane systems of the chloroplast in the diploid black locust were disintegrated and its chloroplast structures were damaged more seriously.On the whole,the ultramicrostructure of chloroplast of tetraploid
出处
《生态学报》
CAS
CSCD
北大核心
2011年第3期734-741,共8页
Acta Ecologica Sinica
基金
国家自然科学基金项目(30901142
31000293)
黑龙江省自然科学基金项目(C201002)
黑龙江省博士后启动基金(LBH-Q09186)
中央高校基本科研业务费专项资金资助项目(DL09BA33)
关键词
四倍体刺槐
二倍体刺槐
叶绿体
超微结构
NaCl和Na2SO4盐胁迫
tetraploid Robinia pseudoacacia
diploid Robinia pseudoacacia
chloroplast
ultramicrostructure
NaCl and Na2SO4
salt stress
