摘要
以卷丹的组培苗为试验材料,研究不同培养方式(固体培养、固-液培养、液体浅层静置培养)、蔗糖与活性炭双因素、茉莉酸甲酯等对卷丹试管内结鳞茎的生长和膨大的影响,以期筛选出对卷丹试管鳞茎生长和膨大有利的培养条件。结果表明:液体培养方式对卷丹试管鳞茎膨大效果较显著,鳞茎的平均鲜质量达到249.84mg,是固体培养条件下的3倍,直径>8 mm鳞茎的产率达54.05%,是固体培养条件下的近5倍;蔗糖和活性炭双因素试验表明,90 g/L蔗糖+3 g/L活性炭是最佳浓度配比,可使鳞茎平均鲜质量达到479.17 mg,最大达到870 mg;茉莉酸甲酯的最佳浓度为10-7mol/L,可使鳞茎平均鲜质量达180 mg;鳞茎移栽对比试验表明:鳞茎越大存活率越高,长势越好;直径>8 mm的卷丹试管鳞茎移栽可以获得较高的存活率及健壮的卷丹植株。
To obtain the suitable culture system of bulblets growth and enlargement,the effects of different culture systems with appending sucrose,AC,Me-JA to the MS medium on the growth and enlargement of Lilium lancifolium bulblets in vitro were studied.The results showed that bulblets are significantly heavy in the liquid culture system compared with solid and solid-liquid.In the liquid culture system,L.lancifolium can culture bulblets with fresh mass of 249.84 mg and percentages of bulblets （diameter〉8 mm） of 54.05% on the average.The concentration of 90.0 g/L sucrose and 3.0 g/L AC was optimal to enlarge bulblets in vitro with the average fresh mass of 479.17 mg and the biggest of 870 mg.The concentration of 10-7 mol/LMe-JA are most favorable for enlargement of bulblets with the average fresh weight of 180 mg.The comparison trials of planting using bulblets of different diameters indicated that the survive of big bulblet were easier than that of small bulblet.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第1期49-53,共5页
Journal of Huazhong Agricultural University
基金
湖北省科技攻关重大项目(No.2006AA205A04)
湖北省自然科学基金项目(2009CDB015)
关键词
卷丹
试管鳞茎
生长
膨大
组织培养
Lilium lancifoliurn
bulblet in vitro
growth
enlargement
tissue culture
