摘要
目的研究亚砷酸钠（NaAsO2）对小鼠肝肾的毒性损伤,探讨亚硒酸钠对亚慢性砷暴露小鼠肝肾的氧化损伤是否具有保护作用。方法 SPF级昆明种小鼠40只,体重18~22 g,按体重随机分为4组：正常对照组、砷模型组、亚硒酸钠对照组、亚硒酸钠＋砷组,分别自由饮用蒸馏水、5 mg/kg亚砷酸钠水溶液、0.8 mg/kg亚硒酸钠的水溶液、5 mg/kg亚砷酸钠＋0.8 mg/kg亚硒酸钠的水溶液,于60 d取材。采用黄嘌呤氧化酶法检测超氧化物歧化酶活性（SOD）、硫代巴比妥法检测丙二醛（MDA）含量,紫外分光法检测髓过氧化物酶（MPO）含量。结果砷模型组小鼠体重有不同程度的减轻,脏器系数升高;亚硒酸钠＋砷组体重、脏器系数均有不同程度的恢复;砷模型组小鼠肝、肾中SOD、MPO含量低于正常对照组,MDA含量高于正常对照组;亚硒酸钠＋砷组小鼠肝、肾中SOD、MPO含量高于砷模型组,MDA含量低于砷模型组。结论亚硒酸钠对砷造成小鼠肝、肾的脂质氧化损伤具有保护作用。
Objective To study sodium arsenite（NaAsO2） on the toxicity of liver and kidney damage in mice,and the effect of sub-chronic arsenic exposure of sodium selenite on oxidative damage in mice liver and kidney.Methods SPF Kunming mice were 40,weigthed 18~22 g,were randomly divided into four groups： Control group,Arsenic in the model group,Sodium selenite group,sodium selenite＋arsenic group.4 groups were free to drink distilled,5 mg/kg sodium arsenite solution,0.8 mg/kg sodium selenite solution,5 mg/kg sodium arsenite＋0.8 mg/kg sodium selenite in aqueous solution,after 60 days,the animal were sacrificed and a series of biochemical indices were detected on organs.The xanthine oxidase activity method,thiobarbituric assay,Penthiobarbital method,ultraviolet spectrophotometry were used to detected superoxide dismutase（SOD）,malondialdehyde（MDA） content,myeloperoxidase（MPO） content.Results Arsenic exposure in mice had different degrees of weight loss,organ coefficient increase;sodium selenite＋arsenic in body weight,organ coefficient had different degrees of recovery;Arsenic group mouse liver,kidney SOD,MPO levels were lower than the control group （P0.05） and MDA content was higher than the control group（P0.05）;sodium selenite＋arsenic in mice liver and kidney SOD,MPO arsenic content were higher than model group（P0.05）;MDA arsenic content was lower than the model group（P0.05）,with significant differences.Conclusion Sodium selenite on arsenic in mice liver and kidney lipid oxidative damage have a protective effect.
出处
《新疆医科大学学报》
CAS
2011年第1期48-51,共4页
Journal of Xinjiang Medical University
基金
新疆医科大学科研创新基金(2008-18)
关键词
砷
硒
氧化损伤
影响
arsenic
selenium
oxidativedamage
impact
