摘要
目的通过对正常人离体白细胞促炎和抗炎处理,观察盐酸戊乙奎醚对人离体白细胞炎性介质分泌的影响。方法严格无菌抽取正常成年人静脉血10ml,加入淋巴细胞分离液,2000r/min离心15min,取出白细胞,加1640培养液使白细胞浓度为106个/ml。平均分为五组,每组6个样本。Ⅰ组:对照组;Ⅱ组:加内毒素;Ⅲ组:加盐酸戊乙奎醚后20min加内毒素;Ⅳ组:加内毒素1h后加盐酸戊乙奎醚;Ⅴ组:加阿托品(与盐酸戊乙奎醚等量)20min后加内毒素。所有组加药后放置培养箱,4h后加肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)检测试剂。结果与Ⅰ组相比,Ⅱ、Ⅲ、Ⅳ、Ⅴ组TNF-α、IL-6、IL-10水平均明显增高;与Ⅱ组相比,Ⅲ、Ⅳ组TNF-α、IL-6、IL-10水平均有不同程度的下降(P<0.01),且Ⅲ组下降程度较Ⅳ组更明显(P<0.05),Ⅴ组TNF-α、IL-6、IL-10水平虽有所变化,但差异无统计学意义。结论抗胆碱药能作用于白细胞非神经性胆碱能受体,抑制白细胞炎性介质的分泌,盐酸戊乙奎醚的作用更明显,尤以提前干预为佳。
Objective To observe the effects of penehyclidine hydrochloride on leukocyte mediators of inflammation through dealing with leucocytes phlegmasia and antiinflammatory in healthy adult.Methods Taken from health adult under asepsis,venous blood samples were add the lymphocyte separating medium,and make it in the density of leucocytes 1×106/ml.The cases were randomly divided into 5 groups（n=6 each）：groupⅠ：control group,group Ⅱ：add endotoxin,group Ⅲ：add endotoxi after adding penehyclidine hydrochloride 20 minutes,group Ⅳ：add penehyclidine hydrochloride after adding endotoxin 1 hour,group Ⅴ：add endotoxin after adding atropine（the same dose with penehyclidine hydrochloride）20 minutes.Place samples of five groups into incubator for 4 hours,then add the TNF-α,IL-10,IL-6 detection reagent.Results The level of TNF-α,IL-6,IL-10 in group Ⅱ,Ⅲ,Ⅳ,Ⅴ were significantly higher than those in group Ⅰ（P〈0.01）.the level of TNF-α,IL-6,IL-10 in group Ⅲ,Ⅳ,especially in group Ⅲ were lower than in groupⅡ,（P〈0.01）.There is no significantly difference between group Ⅱ and groupⅤ（P〈0.05）.Conclusion Anticholine drugs can react on leucocytes cholinergic receptor,inhibit the secretion of leucocytes mediators of infla mmation,the effect of Penehyclidine Hydrochloride is obviously.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2011年第1期24-26,共3页
Journal of Clinical Anesthesiology
