摘要
目的:观察淋巴细胞非神经性AChRs对脾淋巴细胞增殖的调节作用。方法:利用MTT比色法检测AChRs激动剂氨甲酰胆碱对刀豆蛋白A诱导的离体小鼠脾淋巴细胞增殖反应的影响。结果:氨甲酰胆碱在不同的浓度呈现不同的作用,在10-11～10-9mol·L-1和10-5～10-4mol·L-1时显著促进淋巴细胞的增殖,阿托品(10-7mol·L-1)可以阻断该效应。而氨甲酰胆碱(10-7～10-6mol·L-1)可显著抑制淋巴细胞的增殖,美加明(10-7mol·L-1)可以阻断其作用。提示氨甲酰胆碱在10-11～10-9mol·L-1和10-5～10-4mol·L-1可通过激活mAChRs促进淋巴细胞的增殖,而在10-7～10-6mol·L-1通过激活nAChRs抑制淋巴细胞的增殖。结论:氨甲酰胆碱通过激活非神经性mAChRs和nAChRs对淋巴细胞增殖反应产生促进增殖和抑制增殖的双向调节作用。
AIM： To examine the effects of non-neuronal AChRs on the proliferation of splenocytes. METHOD： The effects of AChRs agonist carbachol on the proliferation of mice splenocytes stimulated by concanavalin measured by MTT reduction assay in vitro. RESULTS：The splenocytes responded to carbachol （in the range of 10^-11-10^-gmol·L^-1 and 10^-5 - 10^-4mol·L^-1） byincreased proliferation which were blocked by the muscarinic antagonist atropine （ 10^-7 mol· L^-1 ）. In contrast, carbachol （ 10^-7-10^-6mol·L^-1 ） produced diminished pro-liferation, an effect which were blocked by the nAChRs antagonist mecamylamine （ 10^-7mol·L^-1 ）. It suggests that mAChRs was sensitive to 10^-11 - 10^-9mol·L^-1 and 10^-5 - 10^-4mol·L^-1 carbachol, resulting in increasing proliferation and nAChRs responses to 10^-7-10^-6 mol·L^-1 carbachol leading to proliferation inhibition.CONCLUSION： Carbachol can elicit two different effects according to different concentrations in splenocytes via non-neuronal mAChRs or nAChRs.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第11期1304-1308,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
自然科学基金项目(№30371641)
军事医学科学院创新基金项目。
