摘要
植物雄性不育是植物杂种优势利用的资源,具有重要的生产利用价值。植物雄性不育可从自然突变、人工诱变和远缘杂交中发现,现在可通过细胞工程和基因工程等方法来创造。文章综述了利用基因工程方法制备雄性不育品系及其相应的育性恢复策略,分为"单组分策略"和"双组分策略"。其中利用"单组分策略"制备的不育植株是条件型雄性不育(可逆转的雄性不育),它能在特定的条件下实现雄性可育与不育的转换,实践中可直接作为两用系(不育系和保持系)用于两系法杂交制种;"双组分策略"是利用基因互作和亲本杂交直接培育雄性不育系,或利用基因互作原理分别研制不育系和恢复系,用于三系法生产杂交种。文章分析了"单组分策略"和"双组分策略"的基因工程方法培育雄性不育系及其相应育性恢复策略优缺点,对以上两种技术路线在实际应用中的现状作了分析和展望。
Plant male sterility is a kind of resource of heterosis,which has important value in production.It may be derived from natural mutations,artificial mutations,distant hybridizations,and now through cell engineering and genetic engineering.This paper reviews the progress of strategies in production of plant male sterile lines and their corresponding fertile lines via genetic engineering approach.All strategies can be grouped into ＂single component strategy＂ and ＂two-component strategy＂.＂Single component strategy＂ produces conditional（reversible） male sterile line,whose fertility can be switched under given condition.Conditional male sterile line has two roles,which are CMS and maintainer line for breeding in practice;＂two-component strategy＂ takes advantage of gene interaction and parental hybridization to generate male sterile line.Otherwise,it develops sterile line and restorer line respectively for three-line hybrid system for seed production through gene interaction.This paper discusses the advantages and disadvantages of gene engineering approaches of ＂single component strategy＂ and ＂two-component strategy＂ for developing male sterile line and corresponding restorer line,as well as the current status and perspective of these approaches in practice.
出处
《遗传》
CAS
CSCD
北大核心
2011年第1期40-47,共8页
Hereditas（Beijing)
基金
国家重大基础研究规划项目(973)(编号:2007CB109002)资助
关键词
雄性不育
基因工程
条件型/可逆转的雄性不育
育性恢复
male sterility
genetic engineering
conditional /reversible male sterility
restoring fertility
