摘要
目的:以胶原诱导性关节炎(CIA)大鼠为模型,观察洛伐他汀对CIA大鼠血清细胞因子的影响,为探索炎性关节炎新的治疗药物提供理论基础。方法:利用牛Ⅱ型胶原建立Wistar大鼠CIA模型,造模成功后随机分为空白对照组、CIA模型组、布洛芬组、甲氨蝶呤(MTX)组和洛伐他汀组。分别在第2周和第5周时用ELISA方法检测各组大鼠血清的肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、白介素8(IL-8)和干扰素诱生蛋白-10(IP-10)的水平;用实时荧光定量PCR方法检测第5周时大鼠白介素1β(IL-1β)和白介素17(IL-17)mRNA的表达量。结果:免疫后第5周,洛伐他汀组、布洛芬组和MTX组大鼠的血清TNF-α,IL-6和IP-10的水平显著低于CIA组,而洛伐他汀组和MTX组的IL-8水平与CIA组大鼠无显著差异。洛伐他汀组和MTX组的IL-1β和IL-17 mRNA的表达量显著低于CIA组。结论:洛伐他汀能抑制TNF-α,IL-6,IL-1β,IL-17和IP-10的水平。
Objective： To evaluate effect of lovastatin on serum cytokines in rats with collagen-induced arthritis（CIA） and to supply theoretical basis for exploring new treatments of inflammatory arthritis.Methods： Arthritis was induced by injecting bovine Ⅱ collagen（BIIC） into Wistar rats.The rats were randomly divided into five groups： normal control,CIA control,ibuprofen,methotrexate（MTX） and lovastatin groups.The effects of these agents were assessed by measuring the changes in serum cytokine levels including TNF-α,IL-6,IL-8 and IP-10.The expressions of IL-1β and IL-17 mRNA were detected by QRT-PCR on 5th week.Results： The serum levels of TNF-α,IL-6 and IP-10 in lovastatin group were decreased on 5th week.The expressions of IL-1β and IL-17 mRNA in lovastatin group were less than that of the CIA control group.Conclusion： Lovastatin can decrease serum levels and the expression of TNF-α,IL-1β,IL-6,IP-10,IL-17,MCP-1 and RANTES in rats with CIA.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2010年第24期2319-2322,2327,共5页
Chinese Journal of New Drugs
基金
国家自然科学基金(30771983)
军队十一五课题(08ZL077)
