摘要
目的测定不同大小柚果中柚皮苷的含量。方法采用HPLC法,以甲醇-醋酸-水(35∶4∶61)为流动相,流速为1.5 mL.min-1,检测波长为283 nm。结果柚皮苷的线性范围为2.0～20.0μg(r=0.999 0),柚果中柚皮苷平均含量为169.8 mg.g-1,RSD 1.6%,加样回收率为97.4%,RSD为2.0%,柚皮苷含量随柚果果径增大而下降。结论柚果果径大小可作为控制药材质量的标准之一。
Objective To determine the naringin from different sizes the Citrus grandis（L.） Osbeck.Methods HPLC method was used.Methanol-acetic acid-water（35∶4∶61） was used as the mobile phase.The flow rate was 1.5 mL·min-1,and the determination wavelength was 283 nm.Results The linear range of naringin was 2.0-20.0 μg（r=0.999 0）.The average content of naringin in Citrus grandis（L.） Osbeck was 169.8 mg·g-1 with RSD of 1.6%.The recovery was 97.4% with RSD of 2.0%.The naringin content declined with the increase of the diameter of Citrus grandis（L.） Osbeck.Conclusion The size of Citrus grandis（L.） Osbeck can be used as a quality control criterion for medicinal herbs.
出处
《中南药学》
CAS
2010年第11期839-841,共3页
Central South Pharmacy
