摘要
利用超快速时间分辨光谱研究了蓝绿藻藻胆体的２ 个变藻蓝发射终端（ＡＰＢ， ＡＰＩ）单体和三聚体内的能量传递过程， 探测到ＡＰＢ和ＡＰＩ单体的两组亚基αＡＰ／βＡＰ和αＡＰＢ／βＡＰ（αＡＰ／β１８）间的能量传递时间常数分别为３０ ｐｓ和１９４ ｐｓ． ＡＰＢ和ＡＰＩ三聚体所共有的９～３２ ｐｓ 的短寿命组分来源于同一单体内能量由αＡＰ向βＡＰ的传递过程． 另２ 个短寿命组分（６８ ｐｓ和５０ ｐｓ）则可分别指认为ＡＰＢ内βＡＰ→αＡＰＢ和ＡＰＩ内αＡＰ→β１８能量传递的时间常数， 所测得的长寿命组分则是几种终端基团基态恢复的平衡时间．
Ultrafast time resolved fluorescence spectra were used to study the energy transfer kinetics of API and APB from Cynobacteria Spirulina platensis. The same fast lifetime component(38 ps) was found in API and APB monomers which was attributed to energy transfer between α AP / β AP , another two lifetimes(198 ps,141 ps) observed describe the energy transfer processes of β AP → α APB and α AP / β 18 . In trimers, we also found the excited energy transfer between α AP / β AP in API and APB with 29—32 ps constants. The substitution of α APB in API and β 18 in APB gives rise to a heterogeneity in the chromophore conformations and chromophore chromophore interactions, another fast lifetimes(68 ps and 50 ps) was also found and attributed to the excited energy transfer between β AP / α APB and α AP / β 18 in the same monomers.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第11期1746-1749,共4页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
关键词
藻胆体
变藻蓝蛋白
能量传递
发射终端
Phycobilisome, Allophycocyanin, ps time resolved fluorescence spectra, Energy transfer
