摘要
从螺旋藻藻胆体中分离出4种不同结构和光谱形式的变藻蓝蛋白复合物APⅠ、APⅡ、APⅢ和APB，利用吸收光谱、荧光光谱比较了三聚体和单体的光谱特性，通过对吸收光谱的光谱解曾以及各组分的归属，研究了变藻蓝蛋白复合物内各色团间相互作用的性质和在能量传递中的功能．结果表明，复合物内色团间的作用关系可以用Forster偶极-隅极作用机制来解释，由于连接蛋白和同源亚基的存在影响其结构的对称性，进而影响各色团间相互作用的形式和性质．
Four forms of allophycocyanin (APC ) complexes with different structures andspectral properties in trinleric as well as monomeric aggregates were isolated from phycobili-some and characterized by both absorption and fluorescence spectra. The interactions be-tween chromophores of these APC complexes and their functlons in energy transfer processwere studied by deconvolutlon of absorption spectra. The results indicate that the interactionbetween chromophores of allophycocyanin complexes could be explained by Forster dipoledipole resonance Tnechanism, and it was linker polypeptides and homologous chromophoresthat influence the structural symmetry which flnally affects the interaction mechanism ofchromop hores.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第9期1517-1522,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金!3970065
关键词
变藻蓝蛋白
螺旋藻
藻胆体
APC
PCB
发色团
Allophcocyanin complexes, Fluorescence spectra, Deconvolution of absorption spectra, Dipole-dipole interaction
