摘要
目的探讨人精浆中性α-1,4-糖苷酶(中性α-1,4-G)活性与精子透明质酸酶(HYD)活性(HYD阳性反应率及HYD活性强度)的关系。方法收集年龄24～40岁不育男性精液38例,正常对照12例,根据精浆中性α-1,4-G活性检测结果分为2组:精浆中性α-1,4-G活性正常组(26例)和精浆中性α-1,4-G活性异常组(12例)。24～40岁正常生育男性精液12例作为对照组。用检测盒测定中性α-1,4-G活性,用改良固定底物膜法分别检测精子顶体内HYD阳性反应率及HYD活性强度。结果不育各组中的精浆中性α-1,4-G活性和精子HYD活性(HYD阳性反应率、HYD活性强度)均显著低于正常生育组(P<0.01)。精浆中性α-1,4-G活性与HYD活性(HYD阳性反应率、HYD活性强度)呈显著正相关(r=0.877,r=0.910;P<0.01)。结论精浆中性α-1,4-G活性对精子HYD活性(HYD阳性反应率、HYD活性强度)有明显影响。
Objective To study the relationship among neutral alpha-1,4 glycosidase（ α-1,4-G） activity of human seminal plasma and the positive rate and activity intensity of hyaluronidase （HYD）. Methods Thirty eight samples of semen were devided into 2 groups according to the result of neutral α-1,4-G activity test. Normal birth masculine seminal fluid matched ages was the control group （12cases）. Neutral α-1,4-G activity in semen test kits was used to detect the neutral α-1,4-G activity. The positive rate and activity intensity of HYD in 2 groups were examined by our improved fixed-substrate film method. Results There was significant difference between normal birth group and infertile groups（P 〈0. 01 ）. The relation between neutral α-1,4-G activity and the positive rate of HYD showed a significant positive correlation （r = 0. 877 ,P 〈 0. 01 ） ; the relation between neutral α-1,4-G activity and the activity intensity of HYD showed a significant positive correlation （ r = 0. 910, P 〈 0. 01 ）. Conclusion The neutral α-1, 4-G activity of human seminal plasma may affect the positive rate and activity intensity of HYD.
出处
《基础医学与临床》
CSCD
北大核心
2011年第2期166-169,共4页
Basic and Clinical Medicine
基金
贵州省省长专项基金[黔科教办(2007)03]
贵州省科技厅攻关项目[黔科合S字(2007)1030]
高层次人才科研条件特助经费(TZGF2009-37)
