摘要
[目的]建立同时测定鼠掌老鹳草(Geranium sibiricum)药材中游离和水解原儿茶酸和没食子酸含量的反相高效液相色谱法。[方法]采用安捷伦SBC18色谱柱(4.6 mm×250.0 mm,5μm),以乙腈-0.2%H3PO4水溶液(4∶96,V/V)为流动相,流速为0.8 ml/min,柱温为室温,检测波长为253 nm。[结果]样品中游离原儿茶酸和没食子酸的平均回收率分别为98.42%和97.36%,RSD分别为1.47%和1.56%;水解原儿茶酸和没食子酸的平均回收率分别为99.58%和99.43%,RSD分别为1.79%和1.44%。样品中游离原儿茶酸和没食子酸的含量分别为0.001%和0.041%;水解原儿茶酸和没食子酸含量分别为0.013%和1.387%。原儿茶酸和没食子酸的线性范围分别为0.204～1.020μg(r=0.999 3)和0.078～0.390μg(r=0.999 4)。[结论]该方法可用于鼠掌老鹳草药材的质量控制,方法简便、准确,重现性好。
[Objective]To develop an HPLC method for the simultaneous determination of protocatechuic acid and gallic acid in Geranium sibiricum.[ Method]The Agilent SBC18 column（4.6 mm×250.0 mm,5 μm）was used with mobile phase of acetonitrile-0.2% Phosphate acid（4∶96）.The flow rate was 0.8 ml/min.The detection was set at 253nm and the column temperature at room temperature.[Result]The average recovery was 98.42 % with RSD 1.47 % for free protocatechuic acid and was 97.36 % with RSD 1.56 % for free gallic acid.The average recovery was 99.58% with RSD 1.79 %for hydrolysable protocatechuic acid and was 99.43% with RSD 1.44% for protocatechuic gallic acid.The average content of the samples was 0.001% for free protocatechuic acid and 0.041% for free gallic acid.The average content of the samples was 0.013% for protocatechuic protocatechuic acid and 1.387% for protocatechuic gallic acid.The calibration curve was linear in the range of 0.204-1.020 μg（r= 0.999 3） for protocatechuic acid and 0.078-0.390 μg（r= 0.999 4） for gallic acid.[Conclusion]The method described in this paper is simple and accurate with good reproducibility and can be adopted for the quality control of Geranium sibiricum.
出处
《安徽农业科学》
CAS
北大核心
2010年第34期19352-19353,19355,共3页
Journal of Anhui Agricultural Sciences
基金
通化师范学院自然科学基金项目(201063)
关键词
鼠掌老鹳草
有效成分
含量测定
Geranium sibiricum
Effective components
Determination
