摘要
对水产品中亚硫酸盐含量的测定进行研究,通过比较不同提取和测定方法,改良盐酸副玫瑰苯胺比色法,建立以充氮蒸馏法提取、甲醛溶液吸收、盐酸副玫瑰苯胺比色测定水产品中亚硫酸盐的方法。该方法SO2含量在0.1～2.0μg/mL范围内与吸光度呈良好的线性关系(R2=0.9993),方法的检出限为0.30mg/kg。测定样品的相对标准偏差小于10%,平均回收率在80%～100%之间。该方法重现性良好、使用无毒试剂、回收率能达到检测要求,适用于水产品中亚硫酸盐的测定。
In this study, the determination of sulfite content in aquatic products was investigated. A nitrogen distillation-formaldehyde solution absorption-pararosaniline hydrochloride spectrophotometric method was established through comparing different extraction and determination methods to determine sulfite content in aquatic products. The established method exhibited an excellent linear relationship with correlation coefficient of 0.9993 for sulfur dioxide at the concentration range of 0.1-2.0μg/mL. The recovery rate of this established method was 80%-100% with relative standard deviation of less than 10%. The detection limit of this method was 0.30 mg/kg. Therefore, this developed method is characteristics of good repeatability, less pollution and high recovery rate, which is suitable for the determination of sulfite in aquatic products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期395-401,共7页
Food Science
基金
农业部2008年"水产品中亚硫酸盐的检测"行业标准计划项目(10178)
"十一五"国家科技支撑计划项目(2008BAD94B02)
广东省海洋渔业科技推广专项(A2009001-011(b)
A200901B02
A200901I03)
农业部中央级公益性科研院所基本科研项目(2010YD08
2010TS09)
关键词
充氮蒸馏
盐酸副玫瑰苯胺
水产品
甲醛吸收
亚硫酸盐
nitrogen distillation
pararosaniline hydrochloride
aquatic products
formaldehyde solution absorption
sulfite
