摘要
目的:通过检测乙型肝炎病毒血清中乙型肝炎病毒外膜大蛋白(HBV-LP)、HBV-DNA、乙型肝炎病毒标志物(HBV-M)以及乙型肝炎病毒前S1(Pre-S1),探讨HBV-LP的临床意义及应用价值。方法:采用酶联免疫吸附实验(ELISAI)检测HBV-LP、HBV-M、Pre-S1;采用荧光定量法检测HBV-DNA。结果:在所研究的各种模式中,HBV-LP的阳性率与HBV-DNA的阳性率无明显差异(P均>0.05);HBV-LP阳性率与Pre-S1的阳性率有显著性差异(P<0.05)。HBeAg阴性标本中HBV-LP的检出率63.07%,比HBV-DNA的检出率61.00%无统计学意义;Pre-S1的检出率32.32%则明显低与HBV-LP和HV-DNA。HBV-LP含量与HBV-DNA拷贝数呈正相关性,r=0.950。结论:乙肝患者血清的HBV-LP表达与HBV-DNA拷贝数呈正相关,两者的检出率与符合率均高与Pre-S1,HBV-LP检测可以用于反映乙肝患者病毒复制和抗病毒治疗新的血清免疫学指标,尤其针对HBeAg阴性的患者。HBV-LP检测是一种酶联免疫吸附法,仪器要求低,操作简单,可以在各级医院广泛应用,尤其对于未开展HBV-DNA的医院更具有应用价值。
Objective：To explore the clinical significance and value of the hepatitis B virus large surface protein（HBV-LP） dection by examing hepatitis B virus large surface protein（HBV-LP）,HBV-DNA,hepatitis B virus marker（HBV-M） and hepatitis B virus Pre-S1（Pre-S1） in sero of hepatitis B patients.Methods： Enzyme-liked immunosorbent assay（ELISA） was used to measure the levels of serum HBV-LP,HBV-M and HBV-PreS1,and fluorescence quantitative real-time PCR was used to detect HBV-DNA.Results： The positive rate of HBV-LP and HBV-DNA was no significant difference（P0.05）,and the positive rate of HBV-LP and Pre-S1 was significant difference（P0.05）among various patterns of HBV marker.The detectable rate of HBV-LP（63.07%） was not statistically different from HBV-DNA（61.00%） and the detectable rate of Pre-S1（32.32%） was significantly lower than that of HBV-LP and HBV-DNA in HBeAg-negative HBV patients.There was a positive correlation between the contents of HBV-LP and the copies of HBV-DNA.Conclusion： The expression of the HBV-LP was positively correlated with the copies of HBV-DNA,and the positive rate of the HBV-LP and HBV-DNA was higer than that of Pre-S1,so the HBV-LP detection can reflect the HBV replication and may serve as a new innumo-serological index in the antiviral treatment,especially in the HbeAg-negative patiants.HBV-LP detection was a enzyme-liked immunosorbent assay,low instrument requirement and simple operation,which can be applicated in various hospitals and especially in the hospitals which have not launched the HBV-DNA testing.
出处
《中国卫生检验杂志》
CAS
2010年第12期3394-3396,共3页
Chinese Journal of Health Laboratory Technology
