摘要
目的:建立高效液相色谱法(HPLC)测定卤肉制品中诱惑红的方法。方法:样品用乙醇氨溶液提取,聚酰胺粉净化,高效液相色谱法测定。结果:诱惑红在1μg/ml～20μg/ml范围内有较好的线性关系,仪器最低检出量为:5 ng,方法检出限为:0.25 mg/kg,样品加标平均回收率:82.9%,相对标准偏差:1.69%(n=6)。结论:高效液相色谱法测定卤肉制品中诱惑红,定性准确,灵敏度高,回收率和重现性良好,结果准确可靠。
Objective：To establish a method for the determination of allura red in stewed meat in seasoning based on high performance liquid chromatography（HPLC）.Methods： HPLC is employed for the determination of allura red in stewed meat in seasoning after samples are extracted by ethylate-ammonia solution and clean-upped by polyamide.Results： The standard curve shows good linearity with concentration between 1～20 μg/ml;the method detection limit is 0.25 mg/kg;the average recovery is 82.9% while related standard deviation is 1.69%（n=6）.Conclusion： This method is suitable for the determination of allura red in stewed meat in seasoning.
出处
《中国卫生检验杂志》
CAS
2010年第12期3229-3231,共3页
Chinese Journal of Health Laboratory Technology
