摘要
目的模拟临床上血糖控制不佳出现的糖浓度波动，研究波动性细胞外高糖浓度（ＦＥＨＧ）对肾小球系膜细胞（ＭｓＣ）增殖及其纤维连接蛋白（Ｆｎ）合成的影响。方法利用四甲基偶氮唑蓝（ＭＩＴ）比色法和竞争抑制ＥＬＩＳＡ法动态观察不同葡萄糖浓度及波动性高糖浓度对ＡｓＣ增殖和ＦＮ合成的作用。结果高糖浓度抑制ＭｓＣ增殖，此抑制作用和糖浓度成正相关，同时刺激ＭｓＣ的ＦＮ合成增加；ＦＥＨＧ也抑制ＭｓＣ的增殖，且对ＦＮ的合成有较强的刺激作用。结论细胞外高葡萄糖环境抑制ＭｓＣ增殖，且呈浓度依赖性；波动性细胞外高葡萄糖环境抑制ＭｓＣ增殖，且抗增殖作用大于持续性高葡萄糖浓度组；细胞外高葡萄糖环境和波动性细胞外高葡萄糖均增加ＭｓＣＦｎ的合成，其作用机制尚待进一步研究，波动性高糖浓度对ＭｓＣ的Ｆｎ合成的刺激作用可能在治疗不充分的糖尿病（ＤＭ）患者出现糖尿病肾病（ＤＮ）起重要作用。
Objective: In Order to observe the effects of hyperlgycemia on Mesangial cell(MsC). The effects of fluctuating extracellular high glucose(FEHG) on the proliferation and fibronectin (Fn) synthesis were studied. Methods:The Fn level in supernatant of culturedd MsC and MsC Proliferation were assessed by means of competitive inhibiting ELISA method and MTT colormetric method respectively. Results: High glucose levls inhibited the MsC proliferation and FEHG had the same inhibition effect. the Fn synthesis in FEHG was more higher than that in high glucose level. Conclusion: Fluctuating changes in extracellular glucose concentration may play an important pathological role for FN synthesis of MsC in patients with diabetes mellitus treated inadequatly.
出处
《重庆医学》
CAS
CSCD
1999年第6期412-413,443,共3页
Chongqing medicine
