摘要
组织型纤溶酶原激活物（ｔ－ＰＡ）在人体纤溶中起主导作用，与内皮细胞结合后可提高自身催化活性并免受其抑制物灭活。本实验用联胺诱发内皮细胞过氧化损伤，以硒做为抗氧化剂，用放射性核素测定及放射自显影的方法观察内皮细胞损伤前后与ｔ－ＰＡ结合力的改变。结果表明：过氧化损伤后内皮细胞与ｔ－ＰＡ结合力明显下降，硒对该结合力的下降有一定抑制作用。提示内皮细胞损伤后纤溶活性降低可能与二者结合力下降有关。
issue plasminogen activator (t-PA) plays an important role in fibrinolysis. When t-PA is bound to endothelial cells (EC),its catalytic efficiency is increased and is prevented from being inhibited by its in-hibitor PAI.In this paper,diamine was used to cause injury to cultured human umbilical vein endothe-lial cells by lipoperoxidation,and selenium was used as an anti-oxidant. Radioassay and autoradiography were used to detect the effects of injury on the binding ability of t-PA to EC. The results showed that the binding ability of t-PA to EC was reduced significantly after injury to EC by lipid peroxidation。Sele-nium could inhibit this reduction to a certain extent. It suggests that the decrease of fibrinolysis was re-lated with the decrease of the binding ability of t-PA to EC after EC was injured.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1995年第3期156-158,共3页
Chinese Journal of Pathology
关键词
内皮细胞
损伤
硒
Endothelium,vascular Lipid peroxidation Plasminogen activator,tissue-type
