摘要
目的:探讨保罗样激酶1(PLK1)和p53蛋白在食管鳞状细胞癌(ESCC)组织中的表达及其临床意义。方法:采用免疫组织化学法分别检测87例食管鳞状细胞癌、53例癌旁组织及42例正常食管黏膜组织中PLK1及p53蛋白的表达,并分析两者的表达水平与临床病理因素的关系。采用2χ检验进行统计学分析。结果:PLK1蛋白在食管鳞状细胞癌、癌旁组织及正常黏膜组织中的阳性表达率分别为57.5%、50.9%和26.2%;p53蛋白的阳性表达率分别为48.3%、52.8%和23.8%。鳞癌组织PLK1、p53阳性表达率与正常黏膜组比较差异均有统计学意义(2χ分别为11.119和7.047,P<0.05),癌旁组织PLK1、p53阳性表达率与正常黏膜组织差异均有统计学意义(2χ分别为5.982和8.223,P<0.05),两者在鳞癌组织与癌旁组织的表达差异均无统计学意义(2χ分别为0.567和0.273,P>0.05)。食管鳞癌组织中PLK1和p53的蛋白表达与淋巴结转移及TNM分期密切相关(P<0.05),而PLK1的蛋白表达还与肿瘤浸润深度有关(P<0.01)。两者在食管鳞状细胞癌组织中的表达呈负相关(r=-0.286,P<0.01)。结论:PLK1及p53的蛋白表达与食管鳞状细胞癌发生发展密切相关,联合检测PLK1及p53蛋白对食管鳞癌的预后判断具有重要意义。
Objective： To detect the expression of PLK1 and p53 proteins in esophageal squamous cell carcinoma（ESCC） and to analyze their clinicopathological significance.Methods： The expression of PLK1 and p53 proteins was detected by immunohistochemistry in 87 ESCC specimens,53 tumor-adjacent dysplastic tissue specimens and 42 normal esophageal mucosa specimens.Statistical analysis was performed using the chi-square test.Results： The positive rates of PLK1 and p53 protein expression differed significantly among ESCC,dysplasia and normal esophageal mucosa（PLK1： 57.5%、50.9% and 26.2%;p53： 48.3%、52.8% and 23.8%）.The expression of PLK1 and p53 protein was related with ESCC and normal esophageal mucosa（χ2=11.119 and χ2=7.047,all P0.05）;The expression of PLK1 and p53 protein was related with dysplasia and normal esophageal mucosa（χ2=5.982 and χ2=8.223,all P0.05）;The expression of PLK1 and p53 protein wasn′t related with ESCC and dysplasia（χ2=0.567 and χ2=0.273,all P0.05）.Overexpression of PLK1 and p53 protein was related with lymph node metastasis and TNM stage in ESCC（all P0.05）.PLK1 protein expression was found to be correlated with the depth of tumor invasion（P0.01）.A negative correlation was also noted between the expression of PLK1 and that of p53 in ESCC（r=-0.286,P0.01）.Conclusion： Overexpression of PLK1 and p53 proteins is associated with ESCC invasion and metastasis.Combined detection of PLK1 and p53 protein expression is helpful for evaluation of the prognosis of ESCC.
出处
《江苏大学学报（医学版）》
CAS
2011年第1期58-61,F0003,共5页
Journal of Jiangsu University：Medicine Edition
