摘要
建立测定川楝子中阿魏酸含量的高效液相色谱法。采用VP-ODS C18（250 mm×4.6 mm,5μm）色谱分析柱;流动相为甲醇-1%冰醋酸（25：75）;检测波长：320 nm;流速：0.8 mL/min。结果阿魏酸进样量在0.25~2.50μg范围内与峰面积呈良好的线性关系（r=0.999 6）,平均加样回收率为99.38%,RSD=1.18%（n=6）。该方法简便、准确、可靠,可作为川楝子中阿魏酸的定量分析方法。
HPLC method for the determination of ferulic acid in Melia toosendan was established.The chromatographic separation was performed on a VP-ODS C18 column(250 mm×4.6 mm,5 μm),using methanol-1.0% glacial acetic acid(25:75) as mobile phase.The detection wavelength set at 320 nm and the flow rate was 0.8 mL/min.The results showed that the linear range of ferulic acid was 0.25~2.50 μg,the average recovery rate was 99.38%(r=0.999 6),and RSD was 1.18%(n=6).This method was simple,accurate and reliable.It could be used as the method to determine the contents of ferulic acid in Melia toosendan.
出处
《广州化工》
CAS
2011年第3期11-12,15,共3页
GuangZhou Chemical Industry
基金
国家自然科学基金项目(No:30760299)
关键词
HPLC
川楝子
阿魏酸
HPLC
Melia toosendan
ferulic acid
