摘要
目的分析安徽省涂阳肺结核患者复发患者的基因型别分析,探讨安徽省复发患者形成的原因。方法收集结核病控制项目门诊的患者资料和他们的初始菌株,按国家规划要求治疗,对治愈或完成疗程的涂阳肺结核患者追访2年,并收集复发患者的菌株。对菌株进行菌种鉴定后,采用间隔寡核苷酸分型和多位点数目可变串联重复序列分析[multiple loci VNTR analysis,MLVA]进行基因行鉴定。结果共收取初始菌株351例,追踪249患者,发现复发涂阳患者7例,对符合对比条件的患者5例,经鉴定均为结核分枝杆菌,经Spoligotyping和MLVA分析的结果综合判断,2例为外源性再感染,占40%,3例为内源性复燃,占60%。结论在安徽省复发的病例中外源性再感染和内源性复燃并存。
Objective To determine the cause of smear positive patients of pulmonary tuberculosis.Methods 351 cases and Mycobacterium tuberculosis isolates were collected from the smear positive pulmonary tuberculosis patients of Anhui province.249 cases were cured and follow up 2 years.MTB of relapse of smear positive cases were collected and obtained the genotyped by spoligotyping and MIRU.Results 5 mycobacterium tuberculosis isolates of patients relapse show 3 cases were re-infection and 2 cases were reactivation.Conclusion Re-infection and reactivation were cause of relapse of TB in Anhui province,China.
出处
《安徽医学》
2011年第1期89-91,共3页
Anhui Medical Journal
基金
国家"十一五"重大传染病防治科技重大专项"结核病传播模式"研究项目资助(结核病分子流行病学研究
2008ZX100/03-010-02)
安徽省卫生厅科研基金课题(2002A030)
关键词
分子流行病学
复燃
再感染
肺结核
Molecular epidemiology
Reactivation
Reinfection pulmary
Tuberculosis
