摘要
对植物原料产品和植物源性食品中芥末成分的快速鉴定是避免过敏性疾病发生的重要措施。依据芥末Sin A1管家基因的核酸序列设计特异性引物和探针,对3种芥末样品和21种非芥末植物样品进行实时荧光PCR检测,结果显示,过敏原芥末管家基因的样品FAM通道有荧光信号检出,非芥末样品FAM通道均无荧光信号检出。灵敏度实验表明,植物原料产品中对芥末的检测低限可达到1mg/kg。此外对市售的芥菜籽等样品和深加工的芥末致敏原参考物质(葡萄糖)进行实际样品的检测,均能很好检出致敏原芥末成分。
Rapid identification of mustard materials in plant raw material products and plant-based foods is essential for effective control of a potential source of Allergen pathogens.A convenient Real-Time fluorescence polymerase chain reaction（PCR）-based assay which allowed detection and identification of a mustard-specific Housekeeping gene sin AI DNA sequence in foodstuffs and food was developed.The experiment results show that：the primers and probes could specific identify three kinds of mustard with 21 kinds of samples.Sensitivities results show that：1 mg/kg for mustard of foods could be detected.Moreover,the four commercial samples（including mustard seeds） and deep processing of mustard allergen reference material（glucose） were detected and the detection results show that mustard allergen components were detected well.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2011年第1期61-64,共4页
China Biotechnology
基金
国家公益性行业科研专项课题资助项目(2007GYJ036
10-46)
