摘要
目的 探讨人端粒酶逆转录酶（hTERT）mRNA在胃癌组织中的表达,及其与胃癌生物学行为和根治术后患者预后的关系.方法 应用荧光定量逆转录聚合酶链反应（RT-PCR）检测89例胃癌根治性切除术后患者的肿瘤组织和阴性切缘组织中hTERT mRNA的表达量,对hTERT mRNA表达量与患者的临床病理参数（年龄、性别、肿瘤大小、肿瘤部位、病理类型、组织分化程度、浸润深度、淋巴结转移情况、pTNM分期及生存期）进行单因素分析,采用Cox模型进行胃癌患者生存的多因素分析.结果 hTERT mRNA在阴性切缘组织中的表达量为11.37±2.15,在胃癌组织中的表达量为16.98±3.56,差异有统计学意义（P〈0.05）.胃癌组织中hTERT mRNA的表达量与组织学分级、浸润深度、pTNM分期及淋巴结转移呈正相关（P〈0.05）,与胃癌患者的生存期呈负相关（P〈0.01）.结论 胃癌组织中hTERT mRNA高表达表明胃癌恶性程度较高且病期较晚,检测胃癌组织中hTERTmRNA的表达水平有助于对胃癌患者的预后做出更准确的判断.胃癌组织中hTERT mRNA的表达量、肿瘤浸润深度和pTNM分期是影响胃癌患者预后的重要因素.
Objective By quantitative detection of telomerase expression, we investigated the relationship between telomerase expression and malignant behavior and prognosis in gastric carcinoma.Methods A real-time quantitative RT-PCR （RQ-PCR） was used to quantify the hTERT mRNA copy numbers in 89 samples of gastric carcinoma and corresponding non-cancerous tissues.The clinicopathological data of enrolled patients such as age, sex, tumor size, tumor site, pathologic type, histodifferentiation,infiltration depth, lymph node metastasis, stage and survival were obtained, and were made one factor analysis of variance and COX regression prognostic analysis with those above mentioned markers.Follow-up was completed as of February 28, 2010.The median follow-up was 24 months.Results hTERT from gastric carcinomas and corresponding non-cancerous tissues was 16.98 ± 3.56 and 11.37 ± 2.15, respectively （P〈0.05 ）,the telomerase activity in gastric cancer was significantly higher than that in non-cancerous tissue （ P 〈 O.05 ）.Telomerase activity showed a positive correlation with depth of invasion, tumor differentiation and nodal metastasis （ P 〈 0.01 ）, and negative correlation with survival.Conclusions Gastric cancer with high hTERT mRNA expression indicates a more malignant potential.Detection of hTERT mRNA in gastric cancer may be useful in a better understanding of invasion, metastasis, as well as prognosis of gastric cancer and provide a more efficient therapy.The quantitative expression of hTERT mRNA, infiltration depth and pTNM stage are significant afactors affecting the prognosis of patients with gastric carcinoma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2011年第1期32-36,共5页
Chinese Journal of Oncology
基金
基金项目：贵州省优秀科技教育人才省长基金（1035）
关键词
胃肿瘤
人端粒酶逆转录酶
实时荧光定量逆转录聚合酶链反应
预后
Stomach neoplasms human telomerase reverse transcriptase Real-time fluorescence quantitative PCR Prognosis
