摘要
目的通过与移植法建立的模型对比,探讨开放法子宫内膜异位症模型建立的可行性、判定指标及建模成功率。方法开腹取同一只大鼠的子宫,左侧宫腔采用开放法将子宫内膜与骶韧带或大网膜贴近建模,右侧采用移植法将1.0cm的子宫内膜移植于侧腹膜上建模,1个月后二次开腹判定建模的情况,免疫组化测定骨桥蛋白(OPN)及基质金属蛋白酶-9(MMP-9)的表达。结果开放法建立的模型成模率为81.08%,低于移植法的83.78%,两组比较差异无统计学意义(P>0.05);①成模后肉眼观:移植法以透亮囊肿为主,占64.86%,其次为紫蓝色结节(10.81%),开放法主要以紫蓝色结节为主,占35.13%,其次为透亮囊肿(24.32%);术后盆腹腔粘连开放法器官粘连率为64.86%,明显高于移植法的18.90%(P<0.01);肠梗阻及感染等并发症发生率两组无明显差异(P>0.05);②镜下特点:72.73%的透亮囊肿和68.42%的火焰状改变镜下可见到典型或变异的腺体或腺上皮细胞层,并在异位的腺体及腺上皮细胞上有很强的OPN和MMP-9的表达。结论开放法和移植法均有较高的成模率,但临床观察开放法成模的肉眼观更类似于人类的子宫内膜异位症改变,且具有成模面积大、简单、易行、并发症少等特点。
Objective To research the reliability, evaluation index and achievement ratio of an opening-method model of endometriosis in rats compared with the transplanting-method. Methods Female virginal rats were operated on with the opening-method （the left side of the uterus） and transplanting-method （the fight side of uterus）. In the left side of the uterus, the endometrium was stuck to the uterosacral ligament or omentum majus, while the endometrium of the fight side was implanted onto the fight abdominal wall. The second operation was carried out 4 weeks after implantation to evaluate establishment of the models. Samples from the rats were detected with the osteopontin（OPN） antibody and matrix metalloproteinase-9 （ MMP-9 ） antibody. Results The achievement ratio of the opening-method model was 81.08 %, lower than 83.78 % of the transplanting-method model, with no statistical significance （ P 〉 0.05 ）. The main gross appearance in the transplanting-method model was clear vesicles in 64.80% of all the rats, followed by dark blue nodules in 10.81%. However, the main appearance was dark blue nodules in the opening-method model in 35.13%, followed by clear vesicles in 24.3%. The adherence rate of abdominopelvic organs was 18.9% in the transplanting-method model, significantly lower than 64.86% in the opening-method model （ P 〈 0.01 ）. There was no difference in both intestinal obstruction and infection rates between the two groups （ P 〉 0.05 ）. Microscoptic features ： typical and atypical glands and endometrial cells could be found in 72.70% of vesicles and 68.42% of flame signs, with strong staining of OPN and MMP-9 in the ectopic endometrium. Conclusion The two models both have high achievement ratios. The opening-method model is more similar to human endometriosis in gross appearance, with features of reliability, large area, more convenience and with less complications.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第1期62-66,共5页
Journal of Shandong University:Health Sciences
基金
宁夏科技攻关资助项目(KGX-13-09-06)
宁夏医科大学特殊人才资助项目(XT200914)
