摘要
本文将鱼类抗冻蛋白应用于植物细胞的超低温保存。结果表明,在水稻悬浮细胞的两步法保存中,浓度为0.01mg/ml的抗冻蛋白具有特别的负作用,相对高浓度的抗冻蛋白则能减小细胞存活率的波动性。在玻璃化法保存中,浓度为0.2mg/ml的抗冻蛋白能改善保存效果,更高浓度的抗冻蛋白(>5mg/ml)反而会降低保存效果。环境冰晶量、抗冻蛋白浓度、低温保护剂浓度和细胞膜组成等是影响抗冻蛋白使用效果的几大因素。作者在机理分析中认为,一方面,抗冻蛋白能和冰晶作用,抑制重冰晶,防止去玻璃化;另一方面,抗冻蛋白也能和细胞膜作用,诱发膜表面冰晶形成。
AFP from winter flounder was utilized in cryopreservation of plant cells. During cryop-reservation of rice suspension cells by two-step method, AFP at 0. 01mg/ml damaged the cells extremely. The data obtained at relatively high concentration, however, decreased the variability of survival rate. During vitrification of rice cells, AFP at 0. 2 mg/ml enhanced the viability. However, high concentration AFP (>5mg/ml) decreased the recovery rate. Studies indicated that the results of application of AFP in cryopreservation were closely related to the concentration of
cryoprotectant.The amount of ice crystal in environment, the concentration of AFP and cryoprotectant, and the composition of plasma membrane were several key factors affecting the results of AFP application. In mechanism analysis, the authors suggested that on one hand AFP can interact with ice crystal, which inhibits ice recrystal-lization and prevent the cells from devitrification. On the other hand, AFP also can interact with cell membrane, resulting in the ice growth around the plasma membrane.
出处
《实验生物学报》
CSCD
1999年第3期271-276,共6页
Acta Biologiae Experimentalis Sinica
基金
国家自然科学基金(编号39570083)
浙江省自然科学基金(编号397304)
关键词
抗冻蛋白
超低温保存
水稻
悬浮细胞
Antifreeze proteins. Cryopreservation. Vitrification. Rice suspension cells.
