摘要
报道了水稻根部悬浮细胞固体表面培养的试验结果。由水稻种子根部细胞诱导的愈伤组织,经两次继代培养后进行液体悬浮培养。除培养基成分外,愈伤组织的分裂活性及生理状态,是影响悬浮细胞迅速增殖的重要因素之一.在固体培养基表面培养悬浮细胞,对细胞团的出现和增殖有显著效果.再生小愈伤组织在再分化前进行一次固体继代培养,对再生愈伤组织的增殖和分化有明显的促进作用.
This artieie reports the rice cell suspension cultures on the surface of solid medium. The callus derived from the segments of root of rice(Oryza sativa L.)are used as the initial material of the cell suspension cultures. The results show that cell division activity and physiological condition of callus are two important -factors,- in addition to the compositian of the medium, that affect the growth rate of cells. The cell clusters obtained from the suspension cells on the surtace of solid medium.are favourable to generate plantlets.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
1989年第2期217-222,共6页
Journal of Sichuan University(Natural Science Edition)
关键词
水稻
根部细胞
表面培养
植株再生
enzyme activity, rice, root cell, cell culture, plantlet regeneration.
