摘要
目的:研究补肾益精法对硬皮病成纤维细胞表型的影响。方法:体外培养建立硬皮病成纤维细胞系,制作补肾益精法含药血清干预硬皮病成纤维细胞,使用实时定量RT-PCR技术,检测胶原表达相关因子及甲基化相关基因的表达情况,比较补肾益精法与5-氮杂-2-脱氧胞苷对硬皮病成纤维细胞的作用。结果:补肾益精法可抑制硬皮病成纤维细胞Ⅰ型胶原α_2(Col1α_2)、转化生长因子β(TGF-β)基因的表达,与5-氮杂-2-脱氧胞苷均可提高胶原抑制因子F1i1与Smad7的表达,补肾益精法可减少DNA甲基转移酶1的表达。结论:补肾益精法可改善硬皮病成纤维细胞促纤维化的表型,其过程可能有表观遗传调控机制参与。
Objective： To study the effects of yin-nourishing and marrow-replenishing therapy on scleroderma fibroblast phenotype. Methods： The scleroderma fibroblast cell lines were established in vitro, and were intervened with the medicated serum containing Chinese medicine which has the actions of nourishing yin and replenishing marrow. The expression of related collagen and methylation genes was detected by real-time quantitative PCR. And the effects of yin-nourishing and marrow-replenishing therapy on scleroderma fibroblasts were compared with those of 5-aza-2-deoxycytidine. Results： Yin-nourishing and marrow-replenishing therapy decreased the expression of Col1 α 2 and TGF- β mRNA, DNA methyltransferase 1, and had similar effects on the expression of Flil and Smad7 compared with 5-aza-2-deoxycytidine. Conclusion： Yin-nourishing and marrow-replenishing therapy can counteract the fibrotic processes in the scleroderma fibroblasts, with possible involvement of epigenetic mechanisms.
出处
《新中医》
CAS
北大核心
2011年第2期134-136,共3页
New Chinese Medicine
基金
广东省自然科学基金项目(编号:10451040701005294)
广东省科学技术厅项目(编号:2010B031600050)
关键词
硬皮病
中医疗法
补肾益精法
成纤维细胞
Scleroderma
TCM Therapy
Nourishing-Yin and Replenishing-MarrowTherapy
Fibroblasts
