摘要
目的:探讨切割穹窿海马伞的侧海马提取液对放射状胶质细胞中磷脂酰乙醇胺结合蛋白(PEBP)表达的影响。方法:将培养的海马放射状胶质细胞接种于培养板中,分成对照组和诱导组,对照组中加入含5%正常海马提取液的DMEM/F12细胞培养液,诱导组中加入含5%切割穹窿海马伞侧海马提取液的DMEM/F12培养液。培养7 d后,通过免疫荧光标记法观察PEBP在放射状胶质细胞中的表达,并提取总蛋白,采用Western blot的方法观察对照组及诱导组中PEBP蛋白的表达变化。结果:PEBP蛋白在放射状胶质细胞中能够表达,诱导组的表达比对照组明显增加(P<0.05)。结论:PEBP的表达增加可能与切割侧海马提取液"激活"的放射状胶质细胞向神经元分化有关。
Objective： To observe the phosphatidylethanolamine binding protein（PEBP） expression in radial glia cells which were induced by the extracts from hippocampus after fimbria-fornix transection.Methods： Hippocampal radial glia cells were plated into plates and then divided into control group and induced group.DMEM/F12 medium containing 5% normal extracts from hippocampus was added to the control group,while DMED/F12 medium containing 5% extracts from hippocampus after fimbria-fornix transection was added to the induced group.After 7 d immunofluorescence was used to observe the expression of PEBP in radial glia cells,and then the total protein was extracted and the Western blot was used to observe the expression changes of PEBP protein in radial glia cells.Results： PEBP protein was expressed in radial glia cells,the expression of induced group was significantly higher than that of the control group.Conclusion： The higher PEBP protein expression in the radial glia cells which were activated by the extracts from fimbria-fornix transected hippocampus may be involved with the radial glial cells differentiating into cholinergic neurons.
出处
《南通大学学报（医学版）》
2010年第6期420-422,F0002,共4页
Journal of Nantong University(Medical sciences)
基金
国家自然科学基金资助项目(30470548)
南通市应用研究计划(K2009025)
江苏省高校自然科学研究项目(10KJD180002)
