摘要
通过切割右侧海马伞制备大鼠海马伞损伤模型,应用Westernblotting、免疫组织化学技术,观察切割海马伞后不同时程海马中肾母细胞瘤过度表达基因(NOV)蛋白表达的变化,并对结果进行图像处理和统计学分析。Westernblotting结果显示,NOV蛋白在切割海马伞后3d表达开始上升,14d达高峰后缓慢下降。切割海马伞后14d切割侧和正常侧相比较,海马CA1-CA3区的锥体细胞层及齿状回颗粒层NOV阳性细胞数目无显著性差异(P>0.05),但切割侧NOV阳性细胞明显比正常侧深染,两侧比较平均灰度值有显著性差异(P<0.01)。结合本课题组以往的工作,本研究结果提示,切割海马伞后海马中高表达的NOV蛋白可能参与了诱导神经干细胞迁移和向神经元分化的过程。
The fimbria transected hippocampus model in rats was successfully established by cutting the right fimbria of hippocampus. Using the method of Westem blotting and immunohistochemistry to observe the change of expression of nephroblastoma overexpressed gene （NOV） protein at different times after cutting the fimbria of hippocampus. And image processing and statistical analysis were carried out. The results of Westem blot showed that the expression of NOV protein began to increase 3 days after transaction, came to a head on the 14 day, and then decreased slowly. There were no significant difference about the number of NOV-positive cells in pyramidal layer of CA1- CA3 area and granular layer of dentate gyros between the transected side and the normal side 14 days after transaction （ P 〉 0.05 ）, but the NOV-positive cells in the transected side were much deeper stained than the normal side, and there was significant difference in the average gray values between two sides （P 〈0.01 ）. The present results combined with our previous studies suggest that the over expressed NOV protein in the fimbria transected hippocampus may be concemed with the process of inducing neural stem cells migrating and differentiating into neurons.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2006年第6期624-628,共5页
Chinese Journal of Neuroanatomy
关键词
肾母细胞瘤过度表达基因蛋白
蛋白质印迹
免疫组化
切割海马伞
海马
大田
nephroblastoma overexpressed gene protein, Western blotting, immunohistochemistry, transected fimbria of hippocampus,hippocampus, rats
