摘要
目的:研究香加皮三萜类化合物(triterpenes compounde xtracted from cortex periplocae,TCCP)对荧光素酶(luciferase)标记的人食管鳞癌细胞株Eca109(Eca109-luc细胞)在裸鼠体内成瘤的作用及其机制。方法:Eca109-luc细胞经皮下接种裸鼠,构建Eca109-luc细胞裸鼠移植瘤模型,观察TCPP治疗后移植瘤体积和质量的变化,应用活体成像系统观察移植瘤生长情况,H-E染色观察移植瘤组织形态学变化,流式细胞仪检测移植瘤细胞的凋亡,Westernblotting检测移植瘤组织中survivin的表达。结果:TCPP在体内能明显抑制裸鼠Eca109-luc移植瘤的生长,移植瘤体积和质量明显减少,抑瘤率为40.7%。TCPP治疗组小鼠移植瘤组织出现明显炎性细胞浸润及肿瘤细胞坏死,移植瘤细胞的凋亡率明显高于大豆油对照组(P<0.05),移植瘤组织中survivin蛋白表达水平明显下降(P<0.05)。结论:TCCP在体内能抑制人食管癌Eca109细胞的生长,其作用机制可能与下调survivin的表达诱导肿瘤细胞凋亡有关。
Objective：To investigate the inhibitory effect of triterpenes compound extracted from cortex periplocae （TCPP） on tumorigenesis of luciferase-marked Eca109 cells （Eca109,human esophageal cancer cell line; Eca109-luc） in nude mice and the related mechanisms.Methods： Eca109-luc-implanted tumor model was prepared by subcutaneously injecting Eca109-luc cells into nude mice.After treated with TCPP,the volume and weight of transplanted tumors in nude mice were measured,and tumor growth was monitored with in vivo imaging system.Morphology of transplanted tumor tissues was observed by H-E staining; apoptosis of transplanted tumor cells was analyzed by flow cytometry; and survivin expression in transplanted tumor tissues was examined by Western blotting analysis.Results： The growth of implanted tumor in nude mice was markedly inhibited by TCPP,and the volume and weight of tumors were significantly decreased,with the inhibitory rate being 40.7%.TCPP treatment induced inflammatory cell infiltration and necrosis of tumor cells in implanted tumor tissues,and the apoptosis rate of tumor cells was significantly higher than that in soybean oil control group （P0.05）.Expression level of survivin protein in implanted tumors was significantly lower in TCPP-treated group than that in control group （P0.05）.Conclusion： TCPP can inhibit proliferation of human esophageal cancer Eca109 cells in vivo,which might be associated with the down-regulation of survivin and apoptosis of tumor cells.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2010年第6期620-624,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No.30772752)
河北省自然科学基金资助项目(No.C2008000952)~~
