摘要
目的观察柳茶提取物对非酒精性脂肪肝大鼠肝脏脂质代谢的影响并探讨其作用机制。方法复制非酒精性脂肪肝动物模型;设正常对照组、模型组、西布曲明阳性对照组和柳茶治疗组,药物干预8周后检测各组肝功能、血脂水平及肝匀浆脂肪含量;制作肝组织切片观察形态学变化;实时荧光定量法测肝脏线粒体、微粒体脂质代谢关键酶LCAD、CYP4A1的m-RNA表达水平。结果与非酒精性脂肪肝模型组相比,柳茶治疗后动物肝TG和CHOL水平明显降低;血中ALT、AST含量亦降低,TG、CHOL均有显著下降;而HDL-C水平升高;肝脏脂肪变程度减轻;肝组织LCAD、CYP4A1m-RNA表达水平分别为模型组的4.18、6.04倍。结论柳茶提取物能调节非酒精性脂肪肝动物肝脏的脂肪代谢,减轻肝细胞脂肪变,改善肝功能,其作用机制与上调动物肝脏脂质代谢关键酶LCAD、CYP4A1m-RNA的表达有关。
Objective To investigate the influence of Liucha extractive on lipid metabolism in rats with non-alcoholic fatty liver disease, and to explore its possible mechanism. Methods Rats were divided into normal control group, model group, sibutramine group and Liucha extractive group. Non-alcoholic fatty liver disease rat models were established by feeding with high fat diet. After treatment for 8 weeks, we measured liver function [alanine aminotransferase （ALT） and aspartate aminotransferase （AST） ], serum lipid levels [triglyceride （TG） , cholesterol （CHOL）, high-density lipoprotein cholesterol （HDL-C）, and low-density lipoprotein cholesterol （LDL-C）], and fat level in liver homogenate. HE-stained hepatic tissues were examined to observe the pathological alterations. The m-RNA expression of long-chain aeyl-CoA dehydrogenase （LCAD）and eytomicrosome cytochrome P450 4A1 （CYP4A1）in the ehondrosome and microsome were measured by real-time fluorescent quantitation. Results Compared with the model group, serum TG, CHOL, ALT and AST levels were decreased, homogenate TG and CHOL was lowered（P〈 0.05）, HDL-C was increased（P 〈 0.05） , and liver pathological changes were improved in Liucha group. The expression levels of CYP4A1 and LCAD m-RNA in Liucha group were 6.04 and 4.18 times as much as that in the model group. Conclusion Liucha extractive can regulate lipid metabolism in rats with nonalcoholic fatty liver disease, alleviate fatty changes in liver fat ceils and improve liver function. Its mechanism is related with up-regulating m-RNA expression of key lipid metabolism enzymes of LCAD and CYP4A1.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2011年第1期40-43,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家首届大学生创新计划(860014)
关键词
柳茶提取物
非酒精性脂肪性肝病
脂质代谢
Liucha extractive
Non-alcoholic fatty liver disease
Lipid metabolism
