摘要
目的:探讨安胃汤对实验性慢性萎缩性胃炎大鼠模型的肠三叶因子(intestinal trefoil factor,TFF3)基因表达的影响。方法:采用甲基硝基亚硝基胍(N-methyl-N′-nitro-N-nitroso-guani dine,MNNG)慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠模型,36只清洁级健康雄性Wistar大鼠随机分为病理模型组、安胃汤组、胃复春组共3组。实时荧光定量PCR方法观察安胃汤对实验性慢性萎缩性胃炎大鼠模型胃黏膜TFF3 mRNA表达的影响,对后者进行图像分析及统计学处理。结果:病理模型组,安胃汤组和胃复春组TFF3 mRNA/β-actin值分别为(0.95±0.23),(0.43±0.04),(0.64±0.04),与病理模型组比较,安胃汤组和胃复春组均显著降低TFF3 mRNA/β-actin值(P<0.01);安胃汤组作用优于胃复春组(P<0.05)。结论:安胃汤可能通过降低胃黏膜TFF3 mRNA表达而起到治疗CAG作用。
Objective：To explore the effect of Anwei decoction on the expression of intestinal trefoil factor mRNAs （TFF3 mRNAs） in chronic atrophic gastritis（CAG） rats. Method： Thirty six rats were randomly divided into three groups： model group, Anwei decoction group and Weifuchun group. CAG was induced by N-methy-N-nit ro-N-nitroguanidine（MNNG）. The expression of TFF3 mRNA in rats＇ gastric mucosas was determined by real-time polymerase chain reaction （real-time PCR）. Result： The ratio of TFF3 mRNA/β-actin was 0.95 ± 0. 23,0. 43 ± 0. 04,0.64 ±0. 04 respectively in the model group, Anwei decoction group and Weifuchun group. Compared with that in the model group, the expression of TFF3 mRNA was remarkably reduced in Anwei decoction group and Weifuehun group（P 〈 0. 01 ）. Anwei decoction showed better effect than Weifuehun （P 〈 0.05 ）. Conclusion： Anwei decoction may treat CAG by reducing the expression of TFF3 mRNA in gastric mucosas.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第3期159-162,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西自然科学基金项目(桂科自0728187)
关键词
安胃汤
慢性萎缩性胃炎
肠三叶因子
实验研究
Anwei decoction
atrophic gastritis
intestinal trefoil factor
experimental research
