摘要
目的：建立白斑酊的质量标准。方法：采用薄层色谱法对处方中的栀子、菟丝子、补骨脂进行定性鉴别；采用高效液相色谱测定制剂中的栀子苷含量。色谱条件为：HederaODS-3C18（4．6mm×250mm，5μm）分析柱；乙腈-水（12：88）流动相进行洗脱；流速1．0mL·min-1；检测波长238nm；柱温30℃；结果：薄层色谱鉴别方法专属性强，阴性对照无干扰；含量测定结果表明，栀子苷进样量在0．07620～0．8890μg范围内呈良好的线性关系（r=1．00），平均回收率为97．20％（RSD为2．32％，n=6）。结论：本方法简便、准确、重现性好，可用于白斑酊的质量控制。
Objective： To establish the quality specification of leukoplakia tincture. Methods： Common gardenia fruit, Chinese dodder seed, psoralea corylifolia in this medicine were identified by TLC ; HPLC method was used to determine the content of jasminoidin. And the HPLC conditions were as follows： Hedera ODS -3 C18 （4. 6mm×250mm,5μm） column was served as an analytical column; the mobile phase was methyl cyanides- water （12： 88） with elution at a flow rate of 1.0mL·min-1 ; the detection wavelength was 238 nm and the column temperature was 30℃. Results： The TLC spots were quite clear; The linear range of jasminoidin was within 0. 07620 - 0. 8890μg （ r = 1.00）, the average recovery was 97. 20% and RSD was 2. 32% （ n = 6）. Conclusion： The method is simple, accurate and reproducible, and can be used for the quality control of leukoplakia tincture.
出处
《中国民族民间医药》
2011年第1期55-56,66,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
