摘要
目的:建立胶原诱导性关节炎(C IA)大鼠模型,观察姜黄属常用中药姜黄、郁金、莪术对C IA大鼠滑膜炎症的影响,比较它们的抗炎作用差异。方法:将50只SD大鼠随机分为正常对照组、模型组、姜黄组、郁金组、莪术组。除正常对照组同法注射等量生理盐水外,余各组采用右足趾部皮内注射胶原乳液免疫建立C IA模型。7天后,大鼠尾根部皮内注射胶原乳化液加强免疫1次。初次免疫21天后开始分组给药,姜黄组、郁金组及莪术组分别以中药姜黄、郁金、莪术100%水煎液灌胃;正常对照组、模型组以等量生理盐水灌胃,连续14天。造模后观察各组大鼠的一般情况、关节肿胀程度,计算肿胀率。给药14天后取大鼠左膝关节滑膜组织,固定、包埋、切片、HE染色,观察各组大鼠滑膜组织病理学特征。结果:除正常对照组外,余各组大鼠左足关节明显肿胀,关节肿胀率与正常组比较有显著性差异(P<0.01),说明造模成功;给药14天后,姜黄组、莪术组与模型组比较踝关节肿胀程度减轻,有统计学意义(P<0.05)。病理观察显示,姜黄组、郁金组、莪术组较模型组大鼠滑膜组织充血减轻,炎性细胞浸润减少,以姜黄组最为明显。结论:姜黄属常用中药均能减轻C IA大鼠滑膜炎症反应,其中,以姜黄抗关节炎作用最强。
Objective： To establish CIA rat model and observe the effects of herbs of curcuma genus on synovitis of CIA rats.Methods： A total of 50 rats were randomized into five groups ： Normal control group,model group,Jianghuang group,Yujin group and Ezhu group.Arthritis model was established by injecting collagen and Complete Freund’s Adjuvant（CFA）.After 3 weeks,Jianghuang group,Yujin group and Ezhu group with 100% boiled Jianghuang,Yujin,and Ezhu solution.Control group and model group with equal normal sodium.The size of the swelling joints were measured and compared among groups to define the degree of arthritis grades and the histopathologic features of synovitis tissue of the rats were observed.Results： In model group,the rats′ ankle joint swelling degree increased significantly in comparison with that of rats in Normal control group（P〈0.01）,while the rats′ankle joint swelling degree in Jianghuang group and Ezhu group were lower than that of model group（P〈0.05）.The results of HE staining pathological morphology of synovitis tissue show that the herbs of curcuma genus can inhibit the quantity of inflammatory cell infiltration.Conclusion： All of the herbs of curcuma genus have favorable effects in anti-arthritis and Jianghuang is the most obviously.
出处
《中华中医药学刊》
CAS
2011年第1期95-97,共3页
Chinese Archives of Traditional Chinese Medicine
基金
广东省中医药管理局资助项目(2007334)
广州中医药大学科研创新基金资助项目(K0070006)
