摘要
目的探讨小儿腹腔镜术后感染铜绿假单胞菌(PAE)的耐药性,并检测PAE产β-内酰胺酶现状。方法 PAE采用法国生物梅里埃公司的VITEK-32细菌鉴定系统鉴定到种,药敏试验按CLSI规定的K-B法进行;ESBLs和AmpC酶的表型检测分别采用双纸片扩散试验和头孢西丁三维试验。结果 262株PAE产β-内酰胺酶率为37.4%,其中产ESBLs和AmpC酶率分别为63.3%、36.7%;产酶菌株对常用抗菌药物的耐药率已非常严重。结论对PAE的耐药性监测可帮助临床合理选用抗菌药物,减少细菌耐药性的产生与传播。
OBJECTIVE To approach the drug resistance of Pseudomonas aeruginosa in children after laparoscopic surgery and detect the β- laetamase producing strains. METHODS The isolates species were determined by automated bacterial identification systems, VITEK 32 （bioMerieux, France）. Bacterial susceptibility testing was carried out by Kirby-Bauer methods recommended by CLSI. The phenotype screening for ESBI.s and AmpC enzyme was performed by the double disk method and cefoxitin three-dimensional test, respectively. RESULTS Among 262 strains of PA, 37.4% were β-lactamase producing. The ESBLs and AmpC enzyme producing strains accounted for 63. 3% and 36. 7%, respectively. The drug resistance of enzyme producing strains to common antibiotics was very serious. CONCLUSION The monitoring of drug resistance of PAE can help the rational use of antibacterials and reduce the occurrence and spread of the resistant strains.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第1期148-149,共2页
Chinese Journal of Nosocomiology
关键词
腹腔镜手术
医院感染
铜绿假单胞菌
耐药性
Β-内酰胺酶
Laparoscopic surgery
Nosocomial infections
Pseudomonas aeruginosa
Drug resistance
β-lactamase
