摘要
本文建立了反相高效液相色谱快速测定青蒿中青蒿酸含量的方法。色谱条件为:Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),柱温为(30±1)℃,流动相采用乙腈与0.2%磷酸水溶液混合液(体积比65:35),流速1 mL/min,检测波长220 nm。标准曲线回归方程:Y=8.784573×10-8X-6.443559×10-5,r=0.9997,青蒿酸回收率为102.4%。实验证明该方法稳定可靠、精密度高、重现性好、简单可行,适用于青蒿酸的分析检测。
A method for detecting the content of artemisinic acid which is the key precursor of artemisinin in Artemisia annua L.by RP-HPLC was established.It was carried out in a Diamonsil C18 column（250 mm × 4.6 mm,5 μm） at（30±1） ℃.Acetonitrile combined with 0.2% phosphoric acid aqueous solution （65：35,V/V） was used as the mobile phase at a flow rate of 1 mL/min,and the detection wavelength was 220 nm.The calibration curve equation was Y=8.784573×10-8X-6.443559×10-5（r=0.9997）.A recovery rate of 102.4% was achieved.The results proved that this methed was suitable for detecting artemisinic acid with good stability,high precision,strong reproducibility,and simple operation.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第5期846-849,854,共5页
Natural Product Research and Development
基金
国家高技术研究发展计划(863)基金(2007AA021501)
关键词
青蒿
青蒿素
前体
青蒿酸
反相高效液相色谱
Artemisia annua L.
artemisinin
precursor
artemisinic acid
RP-HPLC
