摘要
对从青蒿母液中提取二氢青蒿酸(DHAA)的工艺进行了优化。以从母液中得到的浸膏总量(提取率)和其中二氢青蒿酸的含量为考察指标,先通过单因素实验对萃取溶剂的种类和浓度、萃取温度,碱液的种类和浓度等因素进行预试,再采用L9(34)正交实验进行系统地考察,以获取最优提取工艺。结果表明:从青蒿母液中提取二氢青蒿酸时,采用乙醇为溶剂时得到的浸膏总量明显高于采用丙酮为溶剂时的浸膏总量;其最佳工艺条件为:萃取溶剂为80%(V/V)的乙醇,萃取温度50℃,采用为1%(w/w)氢氧化钠溶液作为水萃液。在此工艺条件下,母液中二氢青蒿酸的提取率可以达到70%以上,所得样品纯度经HPLC检测可以达到98%。
The extraction process of dihydroartemisinic acid （DHAA） from the remainder of Artemisinin extract was optimized. Taking the yield of extract and the purity of dihydroartemisinic acid in the extract as indexes, the influencing factors such as the types and concentrations of extraction solvents, extraction temperature, the types and concentrations of alkali liquor for increasing the solution pH were investigated, a I9 （ 3 4 ） orthogonal experiment was applied to explore the optimal process. The results indicated that ,the mass of extract obtained by ethanol as solvent was obviously greater than that of acetone as solvent. The optimal process parameters were as follows：applying 80% （V/V） ethanol as solvent and the extraction temperature at 50℃ ;applying 1% （w/w） sodium hydroxide solution as alkali liquor. Under these conditions,more than 70% dihydroartemisinic acid in the remainder of Artemisinin extract was recovered, the purity of the product reached 98% analyzed by HPLC analysis.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第10期1652-1656,共5页
Natural Product Research and Development
基金
中央高校(西南民族大学)基本科研业务费专项资金项目(2014MZDQ05)
西南民族大学研究生创新科研重点项目(CX2015SZ050)
关键词
青蒿
二氢青蒿酸
青蒿素
浸提母液
工艺优化
Artemisia annua L.
dihydroartemisinic acid
artemisinin
extraction remainder
process optimization
