摘要
为寻找一种生产周期短、分离效果好、易操作的纯化黄酮的方法,本研究利用黄酮与金属能形成络合物的性质,从反应体系溶剂、金属盐、解络合剂的添加量优化了络合法纯化银杏黄酮的工艺。结果表明,pH值对络合反应的发生及最终产品的性质具有很重要的影响。金属络合纯化法的最佳工艺条件为:以100 mL甲醇为溶剂,黄酮浓度为2.4 mg/mL,0.20 g硫酸锌为络合剂,反应溶液pH值为9.50,0.2 g EDTA为解络合剂,可以使银杏提取物中黄酮含量由24%提高到55%,得率为58%。
To develop a flavonoid-complex method for purification of flavonoid,the purification conditions for ginkgo flavonoid were studied which included the solvent,the metal,the pH value for complex formation,and the EDTA for decomposition the complex.The results indicated that the pH value was the most important factor to the purification process and the optimized conditions to purify ginkgo flavonoids were as follow：100 mL methanol as solvent,the ginkgo flavonoid concentration was 2.4 mg/ml,0.20 g zinc sulfate as chelating agent,pH value at 9.50,and EDTA（0.20 g） as reagent to decompose complex.After one turn,the flavonoid content was increased from 24% to 55% and the recovery was 58%.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第5期751-754,776,共5页
Natural Product Research and Development
基金
国家"十一五"科技支撑项目(2006BAD12B05)
关键词
银杏黄酮
金属
PH值
络合
纯化
gingko flavonoid
metal
pH value
complex
purification
