摘要
目的观察复合连通多孔钛的体内成骨能力。方法将三维连通多孔钛制成标准试件,浸泡于1.5倍模拟体液中使之复合类骨磷灰石。分别抽取5只新西兰大白兔骨髓各1.5ml,分离出骨髓基质干细胞后进行原代培养。将骨髓基质干细胞接种到多孔钛试件孔壁内,继续培养14d。应用扫描电镜观察成骨情况,然后将其植入兔右侧背肌内,左侧植入空白复合多孔钛试件作为对照。3周后取材进行组织学观察。结果将骨髓基质干细胞接种于复合IPT试件上培养2周后,在光镜及扫描电镜下可见骨髓基质干细胞在孔壁内黏附,增殖活跃。经体内培养3周后的复合多孔钛试件的组织学观察发现,种植有骨髓基质干细胞的复合IPT的孔壁内沉积了一薄层骨组织,而未种植该细胞的对照组样品中未发现有骨组织,其孔隙被大量的纤维组织和少量的肥大细胞等充填。结论预先在体外对复合多孔钛进行细胞接种明显提高了其在体内的成骨效果。
Objective To observe the in vivo osteogenesis of interconnected porous titanium（IPT）.Methods Standard IPT samples were prepared and immersed in a 1.5-fold simulated body fluid to coat bonelike apatite on IPT.Bone marrow（1.5ml） was extracted from 5 rabbits to isolate bone mesenchymal stem cells（BMSC）.BMSC were primarily cultured,inoculated into IPT samples,and cultured for 14 days.Osteogenesis was observed under a scanning electron microscope.Three weeks after BMSC were implanted into the right muscle pockets of rabbits and IPT samples were implanted into the left muscle pockets of rabbits,histological study was performed.Results Two weeks after BMSC were inoculated into the IPT samples,adhesion and active proliferation of BMSC were observed under optical and scanning electron microscope.Three weeks after IPT samples were cultured in vivo,histological study found that a thin layer of bone was deposited in the IPT samples with BMSC but no bone-like tissue in the IPT samples without BMSC.A lot of fibrous tissue and a small number of mast cells were found in holes of the IPT samples.Conclusion BMSC inoculated into IPT in vitro can significantly improve the osteogenesis in vivo.
出处
《军医进修学院学报》
CAS
2011年第1期67-69,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家"863"重大专项课题(2006AA02A136)~~
关键词
连通多孔钛
诱导成骨
骨髓基质干细胞
Interconnected Porous Titanium
Osteogenetic Induction
Marrow Stromal Cells
