摘要
目的:观察还脑益聪方对老年认知障碍大鼠学习记忆能力、脑组织炎性因子含量和海马组织核因子-κB(NF-κB)表达的影响。方法:采用自然衰老大鼠模型,应用Morris水迷宫检测,以青年组大鼠平均逃避潜伏期95%和99%正常值范围上限值为界,筛选认知障碍老年大鼠40只。随机分为还脑益聪方大剂量(13.68g生药/kg)组、小剂量(6.84g生药/kg)组、阳性药物对照(盐酸多奈哌齐,0.45mg/kg)组和老年认知障碍大鼠(模型)组,并设立学习记忆正常的青年大鼠10只、正常老年大鼠10只为空白对照组。灌胃给药8周后,观察大鼠学习记忆能力及脑组织白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,免疫组织化学方法检测海马组织NF-κB的表达。结果:与老年认知障碍大鼠比较,盐酸多奈哌齐组、还脑益聪方组大鼠首次穿越中心的时间显著缩短(P<0.05、P<0.01),在中心区域的寻找平台时间显著加长(P<0.05)。同时,还脑益聪方可以显著降低认知障碍大鼠的IL-6、TNF-α水平(P<0.05,P<0.01),抑制脑组织NF-κB的表达(P<0.05,P<0.01)。结论:还脑益聪方可通过抑制老年认知障碍大鼠的海马组织NF-κB表达,降低脑组织中的炎症因子水平,减轻海马区神经元损伤,达到改善认知障碍大鼠的学习记忆能力。
Objective： To investigate the effect of Huannaoyicong prescription（Polygonum,Ginseng,Coptis,et al,HNYCP） on ethology,expression of NF-κB（nuclear transcription factor-κB,NF-κB）in hippocamp tissue and the levels of inflammatory factor which are the indicators of brain tissue of cognitive impairment rat Methods：Fourty cognitive impairment rats were screened adopting nature senile model of rats and the behavior test method of Morris water maze,To the average escape latency of the young rats were several times the standard deviation of ＋2 Linear limit＋1 Linear limit the standard deviation of maximum.All of the rats were randomly divided into the following four groups：large dose group of HNYCP treatment（13.68g crude drug per kilogram）;low dose group of HNYCP treatment（6.84g crude drug per kilogram）;active control group of which the active drug was donepezil hydrochloride（0.45mg per kilogram）;cognitive impairment group,of each group there were ten rats.Meanwhile,10 young rats and 10 ageing rats with normal learning and memorial ability were equally set as two blank control groups.Observed learning and memorial ability of rats and tested levels of IL-6 and TNF-α in brain tissue and NF-κB expression in hippocamp tissue with immunohistochemistry method after 8 weeks＇ intragastric administration.Results： Compared with the cognitive impairment group,the first crossing time of rats on central area reduced significantly（P0.05,P0.01）,the searching time on central area extended significantly（P0.05）in active control and HNYCP treatment groups.Meanwhile,the levels of IL-6,TNF-α in brain tissue of cognitive impairment rats decreased significantly（P0.05,P0.01）,the positive expression of NF-κB in hippocamp tissue had been inhabited significantly in HNYCP treatment groups（P0.05,P0.01）.Conclusion： HNYCP can reduce the injury of hippocampal neurons furthermore improve the learning and memorial ability of cognitive impairment rats in the way of inhibiting NF-κB expression in hip
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第1期156-160,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家中医药管理局科技专项课题(No.04-05JP65)
国家自然科学基金创新研究资助项目(No.30672571)
国家科技重大专项课题~~
关键词
还脑益聪方
老年性痴呆
认知障碍
学习记忆
炎性因子
核转录因子-ΚB
老年大鼠
Huannaoyicong Prescription
Alzheimer disease
Cognitive impairment
Learning and memorable ability
Inflammatory factors
NF-κB
Old rat
