摘要
目的:探讨维药罗勒不同提取组分在体外对环氧化酶-1(COX-1)的合成物6-keto-PGF1α浓度的影响,间接证明其对COX-1活性的影响,进而找出该药抗血栓的作用机制及其有效部位。方法:将维药罗勒不同提取组分对Ecv304细胞进行不同时间的药物干预,采用MTT法检测活细胞数目,ELISA法测定细胞上清液中6-keto-PGF1α的浓度。结果:不同组分在不同时间内对6-keto-PGF1α的浓度有不同的影响,在短时间内,OBL-正丁醇和OBL-乙醇均能显著增加6-keto-PGF1α的浓度(P<0.01);长时间内,OBL-乙酸乙酯可显著性地降低6-ket-PGF1α的浓度(P<0.05),而OBL-氯仿和OBL-正丁醇中高剂量可显著增加6-keto-PGF1α的浓度(P<0.01)。结论:维药罗勒可能通过抑制TXA2合成酶的活性,激活COX-1的酶活性,最终维持TXA2-PGI2的平衡而产生抗血栓作用,正丁醇提取部位是其发挥激活COX-1酶活性作用的有效部位。
Objective： To study the effects of different polar extract of Uighur Ocimum basilicum L.on COX-1 activity in ECV304 cells in vitro,and find its anti-thrombus mechanism and effective parts.Methods： Ecv304 cells was interfered with different polar extract of Uighur Ocimum basilicum L.in different time.The number of living cells was detected by MTT method.And the concentration of 6-keto-PGF1αin supernatant was analyzed by ELISA method.Results： There are different effects of different polar extract on the concentration of 6-keto-PGF1α in different time.In short,OBL-n-butyl alcohol and OBL-ethanol can significantly increase the concentration of 6-keto-PGF1α（P0.01）;a long time,OBL-ethyl acetate can significantly reduce the concentration of 6-keto-PGF1α（P0.05）,whereas OBL-chloroform and OBL-n-butyl alcohol in middle and high doses can significantly increase the concentration of 6-keto-PGF1α（P0.01）.Conclusion： Uighur Ocimum basilicum L.may inhibit the activity of TXA2 enzyme and activate the activity of COX-1 enzyme.And then the balance between TXA2 and PGI2 will be maintained which can cause anti-thrombus.And the extract part of n-butyl alcohol is one of effective parts when activating the activity of COX-1 enzyme.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第1期150-152,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金(No.30660210)~~
关键词
罗勒
环氧化酶-1
抗血栓
细胞
有效部位
酶活性
Ocimum basilicum L.
COX-1
Anti-thrombus
Cells
Effective parts
Enzyme activity
