摘要
黄酒酵母的耐受性对黄酒酿造至关重要。对原始菌株YS分别采用乙醇-热冲击法、细胞紫外诱变法和原生质体紫外诱变法,结合高温驯育进行选育,筛选到一株在38℃能正常生长发酵的黄酒酵母突变株YS6.2.5。YS6.2.5体积小,近似圆形,产香好,产酸少,30℃时产酒精能力、酒精耐性、高糖耐性分别为9.0%、19%、30°Bx,38℃时相应指标分别为6.5%、20%、28°Bx。酿酒实验表明,其生理耐性强,酿酒性能好,遗传性状稳定,具有较好的工业应用前景。
Tolerance of rice wine yeast is essential for brewing rice wine. In order to breed tolerant rice wine yeast, original yeast strain YS （Saccharomyces cerevisiae） was subjected to ethanol-heat-shock, UV-induced mutagenesis of cells and proptoplasts coupled with high temperature domestication to screen and obtain a mutant yeast YS6.2.5 with normal growth at 30 ℃ for rice wine fermentation. The mutant YS6.2.5 had the characteristics of small size, round shape, good aroma-producing favor and low acid-producing property. The alcohol-producing capability, alcohol tolerance, sugar tolerance of YS6.2.5 were 9.0%, 19% and 30 °Bx at 30 ℃, and 6.5%, 20% and 28 °Bx at 38 ℃, respectively. Fermentation tests showed that the YS6.2.5 had strong physiological tolerance, high fermentation power, stable hereditary, and better industrial application prospect.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第23期228-232,共5页
Food Science
关键词
黄酒酵母
耐受性
诱变育种
热效应
原生质体
rice wine yeast
tolerance
mutagenesis breeding
thermal effect
protoplast
