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盐胁迫下梭梭幼苗膜脂过氧化和保护酶活性 被引量:12

Salt stress on membrane lipid peroxidation and protective enzymes in Haloxylon ammodendron seedling
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摘要 使用0、50、100、200、350、500 mmol/L NaCl处理梭梭幼苗,分三次取样,并测其相应指标,结果表明梭梭幼苗细胞膜透性在10天时保持稳定;当胁迫到20天、30天时,同化枝膜透性随盐浓度的增加及胁迫时间的延长逐渐增加。整个试验过程中,丙二醛含量逐渐增加,但前期增加幅度不大,后期梭梭幼苗同化枝的丙二醛含量在NaCl胁迫30天时为2.569μmol.g-1。SOD和POD活性在整个试验过程中相对于对照而言整体呈增加趋势,但在前期增加得较快,后期较慢。CAT活性在整个过程中则一直呈增加趋势。 This paper studied Haloxylon ammodendron seedlings were treated by NaCl stress with concentrations of 0,50,100,200,350 and 500 mmol/L. Samples were extracted by three times and measure its data after NaCl stressed 10 days, 20 days, 30 days. It turned out that the membrane permeability kept stable within 10 days. The membrane permeability of assimilation branch was increasing with the increasing of the NaCl stress and the time protracting to 20 days and 30 days. When NaCl concentrations reached 350 mmol/L, membrane permeability of 20 days has significant difference, membrane permeability of 30 days has notable difference. In this experiment, malondialdehyde content was increasing gradually although its accession range was little in earlier stage, the malondi aldehyde content of Haloxylon ammodendron assimilation branch was 2.569 μmol/L by 500 mmol/L NaCl stress in 30 days. It showed that the lipid peroxidatic reaction level was not significant, cell membrane system was damaged to a certain extent. SOD and POD activities of Haloxylon ammodendron assimilation branch was increasing in the whole treatment, but it increased faster in earlier stage and slower in late stage. Its activity reached maximum when NaCl concentration was 350 mmol/L and stressed 10 days and 20 days. CAT activity displayed increasing tendency in the whole course. It showed that Haloxylon ammodendron seedlings' cell membrane system would damaged only after the stress time and stress concentration combined action, which also explained Haloxylon ammodendron's salt endurance.
出处 《干旱区地理》 CSCD 北大核心 2008年第6期904-909,共6页 Arid Land Geography
基金 国家自然科学基金(30760200) 教育部2006年度优秀人才支持计划项目(NCET-06-0919)
关键词 盐胁迫 梭梭 MDA SOD POD salt stress Haloxylon ammodendron malondialdehyde superoxide dismutase peroxidase
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